Microbial biotransformation of medroxyprogesterone / Maisarah Abdul Aziz

• Main Author: Abdul Aziz, Maisarah
• Format: Thesis
• Language: English
• Published: 2014
• Subjects: Pharmacopoeias
• Online Access: https://ir.uitm.edu.my/id/eprint/111224/1/111224.PDF; https://ir.uitm.edu.my/id/eprint/111224/

Microbial biotransformation studies are very important in a new drug discovery or in pharmaceutical products for many years. The advantages of this process compare to chemical synthesis method were that, this method has high stereoselectivity, enantiomeric, regioselective under mild conditions, handle easily, cheaper and green environment by reduced the hazardous chemical usage. In the current work, the compound of interest or starting compound used to undergo the biotransformation process is medroxyprogesterone (MP), which was derived from steroids and also an metabolite of medroxyprogesterone acetate (MP A). MP was undergo fermentation with fungi for different incubation times. The extract obtained after fermentation were then analyzed by using High Performance Lipid Chromatography (HPLC) with Diode Array Detector (DAD). There are seven fungus involved in this studies which were C. elegans, V. lecanii, A. coerulea, B. bassiana, B. cinera, T. roseum, and M plumbeus. Lastly, the HPLC profiles for each fungus and startingcompound were compared and the results obtained showed that, medroxyprogesterone may have the possibility to undergo biotransformation with C. elegans, A. coerulea, B. cinerea, T. roseum, and M plumbeus only by showing significant results in HPLC.