Development of HPLC assay method for gamma-linolenic acid / Noor Izzati Filza Salihoudin

• Main Author: Salihoudin, Noor Izzati Filza
• Format: Thesis
• Language: English
• Published: 2013
• Subjects: Pharmaceutical chemistry
• Online Access: https://ir.uitm.edu.my/id/eprint/110490/1/110490.PDF; https://ir.uitm.edu.my/id/eprint/110490/

Reverse phase high performance liquid chromatography (RP-HPLC) method using UV detector was developed for analysis of gamma-linolenic acid (GLA). Zorbax 300SB C-18 (5 µm, 250 x 4.6 mm internal diameter) column was the stationary phase and 90% (v/v) acetonitrile and 10% (v/v) deionized water were employed as the mobile phases in an isocratic manner. The detection was done by using UV detector at 211 nm wavelength with the flow rate of 0.8 ml/min. Calibration curves for GLA were linear with average correlation coefficient 0.995. The coefficient of variation for interday analysis were all less than 1 % except for day 2. This study was able to have a great reduction in retention time in comparison to the previous research finding.