Apoptotic activity of pineapple honey (ananas comosus) towards MCF-7 breast cancer cell lines / Maidrah Mahdzan

• Main Author: Mahdzan, Maidrah
• Format: Thesis
• Language: English
• Published: 2013
• Subjects: Pharmacopoeias; Pharmacognosy. Pharmaceutical substances (Plant, animal, and inorganic)
• Online Access: https://ir.uitm.edu.my/id/eprint/110337/1/110337.PDF; https://ir.uitm.edu.my/id/eprint/110337/

Pineapple honey is a Malaysian honey with great benefits which act as an anticancer, antioxidant to prevent oxidative damage, anti-inflammatory agent and others. This study focused on the apoptosis ultra-structural changes of the MCF-7 breast cancer cell lines by the induction of the pineapple honey. Pineapple honey with the properties as an antioxidant such as phenolic compounds and flavonoids content was also discussed. TPC and TFC assays were performed to generate values for total phenolic content and total flavonoids content of pineapple honey. Much emphasize given to transmission electron microscopy method to study on the morphological changes of the apoptosis on MCF-7 which was induced by pineapple honey. Cell shrinkage, plasma membrane blebbing, chromatin condensation and fragmented DNA were the main features of apoptosis observed in the study. Three different concentrations (2.5%, 5%, 10%) of the pineapple honey were tested at optimum 48 hours incubation time, and 10% concentration was the best to present the apoptotic cell changes. The results acquired were proof to why pineapple honey is also a suitable candidate of antioxidant thus an anticancer to fight against the oxidative stress to cure breast cancer.