Detection of acinetobacter baumannii using polymerase chain reaction / Noradila Shahrom
Acinetobacter baumannii is a multidrug resistant bacterium and a major pathogen causing nosocomial infections worldwide, including pneumonia, wound infections, urinary tract infections, bacteremia and meningitis. A rapid, sensitive and specific detection method is needed for cases involving Aibauman...
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| Main Author: | |
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| Format: | Thesis |
| Language: | English |
| Published: |
2013
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| Subjects: | |
| Online Access: | https://ir.uitm.edu.my/id/eprint/109501/1/109501.PDF https://ir.uitm.edu.my/id/eprint/109501/ |
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| Summary: | Acinetobacter baumannii is a multidrug resistant bacterium and a major pathogen causing nosocomial infections worldwide, including pneumonia, wound infections, urinary tract infections, bacteremia and meningitis. A rapid, sensitive and specific detection method is needed for cases involving Aibaumannii. Thus, the objective of this study is to develop a diagnostic test, which is PCR for the detection of Aibaumannii. The primers were designed for Conserved gene 1, Conserved gene 2 and ada[H] gene. Results showed that the detection of those genes was successful. Through visualization of gel electrophoresis result for PCR products, DNA bands for Conserved gene 1, Conserved gene 2, ada[H] gene and multiplex PCR were observed. This showed that those genes were present in Abaumannii and were successfully detected using the developed diagnostic test. The detection of A.baumannii using the novel primers was successful. PCR assay can be applied as a robust, rapid, sensitive and specific detection method for Abaumannii. |
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