Development and validation of HPLC method for analysis of cocaine for extemporaneous preparation stability test / Siti Noriah Muhd Yuna
Cocaine is an addictive and powerful drug which has been used mainly as anesthetic agent. The objective of this study is to develop and validate method for quantitative analysis of cocaine for extemporaneous preparation stability test using high performance liquid chromatography (HPLC). High perform...
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| Main Author: | |
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| Format: | Thesis |
| Language: | English |
| Published: |
2013
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| Subjects: | |
| Online Access: | https://ir.uitm.edu.my/id/eprint/109266/1/109266.PDF https://ir.uitm.edu.my/id/eprint/109266/ |
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| Summary: | Cocaine is an addictive and powerful drug which has been used mainly as anesthetic agent. The objective of this study is to develop and validate method for quantitative analysis of cocaine for extemporaneous preparation stability test using high performance liquid chromatography (HPLC). High performance liquid chromatography with diode array detector was used to develop the method and multiple wavelength detector was used in order to validate the developed method. Chromatography was performed on ZORBAX 300SB-Cl8 (4.6 mm x 150 mm, 5µm particle size, Zorbax) using acetonitrile phosphate buffer pH 4 as mobile phase with the flow rate of lmL/min. A linear calibration curve over concentration range of 10 µg/mL until 320 µg/mL was obtained. The average correlation coefficient (R2) for cocaine was 0.9994. The accuracy was founded to be satisfactory. |
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