Effects of crude and fraction (C3) endophytic aspergillus sp. HAB10R12 on the TNF-α induced - insulin resistance differentiated 3T3-L1 adipocytes / Nur Amalina Yahaya

• Main Author: Yahaya, Nur Amalina
• Format: Thesis
• Language: English
• Published: 2013
• Subjects: Pharmaceutical chemistry
• Online Access: https://ir.uitm.edu.my/id/eprint/109257/1/109257.PDF; https://ir.uitm.edu.my/id/eprint/109257/

Endophytes Aspergillus sp strain HAB10R12 isolated from tree Garcinia scortechinii (kenondong krot @ kandis) exert many beneficial effects such as anticancer, antimicrobial, and anti-diabetic. However, the study on the antidiabetic of this compound is still limited. In the present study, we analysed the possible effect of the Endophytes Aspergillus using the 3T3-L1 cells as an in vitro cellular model. MTT assay has confirmed that the endophyte extracts do not cause cytotoxic effect on 3T3-L1 adipocytes since there is no IC-50 after 24 hours exposure. However, endophytes may or may not stimulate the glucose uptake 3T3-L1 adipocytes. The observation on glucose uptake demonstrated that endophytes had varying ability to take up glucose in the insulin resistance induced 3T3-L1 adipocytes with TNF.