Culture of penicillium sp strains F23 with and without DMSO and isolation of its secondary metabolites / Ahmad Nasrun Mohd Daud

• Main Author: Mohd Daud, Ahmad Nasrun
• Format: Thesis
• Language: English
• Published: 2013
• Subjects: Pharmacopoeias; Pharmaceutical chemistry
• Online Access: https://ir.uitm.edu.my/id/eprint/109144/1/109144.PDF; https://ir.uitm.edu.my/id/eprint/109144/

The study is done to investigate the effect of volatile toxic compound which is Dimethyl Sulfoxide (DMSO) on the production of secondary metabolite of Penicillium isolated from soil with DMSO. The study was done by growing the fungi in the presence and absence of DMSO. The cultures were incubated for a two-week period at room temperature and extraction of the fungi metabolites were performed using ethyl acetate. High performance liquid chromatography (HPLC) analysis was performed on the extracts. The chromatograms were compared between samples obtained in the presence and absence of DMSO. 10 peaks that showed higher concentration were collected. The 10 collected peaks will be further studied by post-graduate students to determined the structure elucidation by using various spectroscopy analysis such as Nuclear magnetic resonance (NMR), Liquid chromatography-mass spectrometry (LC-MS) and Infrared radiation (IR).