Development of a PCR method for the detection of polymorphism of apolipoprotein E gene / Nur Farhana Mohamed Noor
Apo E gene plays an important role in producing apolipoprotein E that serves as a ligand for uptake by lipoprotein receptor so that cholesterol can be removed from the body. Screening of Apo E gene is important before starting drug therapy as different patient will act differently to the statin ther...
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| Format: | Thesis |
| Language: | English |
| Published: |
2009
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| Online Access: | https://ir.uitm.edu.my/id/eprint/107168/1/107168.PDF https://ir.uitm.edu.my/id/eprint/107168/ |
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| Summary: | Apo E gene plays an important role in producing apolipoprotein E that serves as a ligand for uptake by lipoprotein receptor so that cholesterol can be removed from the body. Screening of Apo E gene is important before starting drug therapy as different patient will act differently to the statin therapy. The aim of this study is to develop and validate a PCR method for detection of polymorphism of apo E gene. Allele specific PCR together with nested PCR have been used. Two separate amplification are used. First PCR used a set of primers that yield a large product which was used as a template in the second PCR to enhance the sensitivity and specificity of DNA. The PCR was conducted under different annealing temperature to determine the optimal annealing temperature which is 61 °C for the first PCR and 57.6°C for the second PCR. |
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