Synthesis of single-stranded RNA marker / Abdul Shukor Said
Single-stranded RNA marker is widely used in biotechnology field. Producing it on our own will reduce the laboratory cost. This study aimed to synthesis single-stranded RNA marker. In this research, two method were used which are PCR process and followed by in vitro transcription. PCR is a special t...
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| Main Author: | |
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| Format: | Thesis |
| Language: | English |
| Published: |
2009
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| Subjects: | |
| Online Access: | https://ir.uitm.edu.my/id/eprint/107107/1/107107.PDF https://ir.uitm.edu.my/id/eprint/107107/ |
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| Summary: | Single-stranded RNA marker is widely used in biotechnology field. Producing it on our own will reduce the laboratory cost. This study aimed to synthesis single-stranded RNA marker. In this research, two method were used which are PCR process and followed by in vitro transcription. PCR is a special technique to amplify the DNA sequence of interest. A successful laboratory testing for PCR depends on sensitivity and specificity of PCR method itself. In vitro transcription is a technique to transcribe double stranded DNA to single stranded RNA. Ten different size of DNA ranging from 1 kb to 10 kb were amplified and undergo in vitro transcription to synthesis single stranded RNA with the same bases range. |
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