Detection of protein changes in female reproductive system of dose response bisphenol A treated SD juvenile rats / Aizamin Anuar

• Main Author: Anuar, Aizamin
• Format: Thesis
• Language: English
• Published: 2009
• Subjects: Pharmaceutical industry; Pharmacognosy. Pharmaceutical substances (Plant, animal, and inorganic)
• Online Access: https://ir.uitm.edu.my/id/eprint/107032/1/107032.PDF; https://ir.uitm.edu.my/id/eprint/107032/

Bisphenol A or BPA are used in the production of epoxy resins and polycarbonate plastics. Other applications of BPA are in baby bottles, canned food and drinks and polymers contained in dental treatment. BPA is a type of xenoestrogen that has been shown to mimic the actions of estrogen. Particularly in females, BPA causes ovarian disease, stimulation of mammary gland, onset of sexual maturation and disruption of the estrous cycle. Other than that, BPA can also stimulate human breast cancer cell. The dose and the effects of BPA on the reproductive system vary. The effects of BPA on the early onset of sexual maturation in females was seen at 2.4 and 500µg/kg/day, the stimulation of mammary gland in female offspring occurred at very low dose of 0.025µg/kg/day and BPA disrupts the adult estrous cycle at the dose between 100 and 500µg/kg/day. To determine the concentration of protein in female rats, we force-feed the rats with dose of BPA ranging from 50, 500, 1000 and 5000µg/kg/day and using SOS PAGE to observe the intensity of the concentration of protein in each sample. In our study, the concentration of proteins in the group of rats fed with 50 and 500µg/kg/day increased above the concentration of proteins in the negative control groups especially in the group with the 500µg/kg/day dose which was almost similar to concentrations detected in the positive control. However, at 1000µg/kg/day, there was almost no protein and at 5000µg/kg/day the concentration of protein was similar to the negative control group. Based on the weight of ovary, the negative control and the group fed with 1000 and 5000µg/kg/day had increased weight compared to the other groups which shows that a lower concentration of protein correlates with increased of ovary weight. However, further analysis using Western Blot needs to be carried out in the future so that the specific band and concentration for estrogen and progesterone could then be observed and analyzed.