Development of a PCR based method for detection of polymorphism of monoamine oxidase a gene / Nurfaliyana Wahab

• Main Author: Wahab, Nurfaliyana
• Format: Thesis
• Language: English
• Published: 2009
• Subjects: Pharmaceutical industry; Pharmaceutical chemistry
• Online Access: https://ir.uitm.edu.my/id/eprint/106937/1/106937.PDF; https://ir.uitm.edu.my/id/eprint/106937/

Monoamine oxidase A (MAOA) enzymatically degrades the biogenic amme neurotransmitters norephinephrine, dopamine, and serotonin that plays critical role in regulation of their transmission. The MAOA gene has been associated with several behavioral disorders such as antisocial, aggressive impulse disorder and many more. When there are polymorphisms at the gene site, this may lead to drug toxicity and drug abuse. In order to identify the polymorphism of the gene, we can use the Polymerase Chain Reaction (PCR) and Gel Electrophoresis (GE). The aim of this study is to design the DNA primers to detect the MAOA allele and also to develop optimized PCR based method for detection polymorphism of MAOA. This study aims to design the specific primers and optimized the PCR method detection of polymorphism of MAOA gene. Based on the result, the DNA sample have low chances of polymorphism in MAOA gene that can lead to several behavioral disorders.