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Synthesis of high molecular weight DNA ladder / Dayana Abdul Bari

DNA ladder has been used as a tool in laboratories to determine the size of DNA fragment. The aim of this study is to synthesize high molecular weight DNA ladder and to optimize PCR reaction in order to obtain high yield and specific product. High molecular weight DNA ladder ranges from 0.5 - 10 kbp...

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Bibliographic Details
Main Author: Abdul Bari, Dayana
Format: Thesis
Language:English
Published: 2009
Subjects:
Pharmaceutical industry
Botanical chemistry. Phytochemicals
Online Access:https://ir.uitm.edu.my/id/eprint/105530/1/105530.PDF
https://ir.uitm.edu.my/id/eprint/105530/
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spelling my.uitm.ir.1055302024-12-05T08:48:42Z https://ir.uitm.edu.my/id/eprint/105530/ Synthesis of high molecular weight DNA ladder / Dayana Abdul Bari Abdul Bari, Dayana Pharmaceutical industry Botanical chemistry. Phytochemicals DNA ladder has been used as a tool in laboratories to determine the size of DNA fragment. The aim of this study is to synthesize high molecular weight DNA ladder and to optimize PCR reaction in order to obtain high yield and specific product. High molecular weight DNA ladder ranges from 0.5 - 10 kbp. DNA ladder is synthesized by using Polymerase Chain Reaction (PCR) method, where lambda phage DNA is used as a template for both PCR reaction and designing primer. The results showed that high molecular weight DNA ladder could be synthesized in laboratory setup. Thus, it could be used as a laboratory tool in the future and may reduce the cost incurred by the faculty in carrying out related studies. 2009 Thesis NonPeerReviewed text en https://ir.uitm.edu.my/id/eprint/105530/1/105530.PDF Synthesis of high molecular weight DNA ladder / Dayana Abdul Bari. (2009) Degree thesis, thesis, Universiti Teknologi MARA (Kampus Puncak Alam).
institution Universiti Teknologi Mara
building Tun Abdul Razak Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Teknologi Mara
content_source UiTM Institutional Repository
url_provider http://ir.uitm.edu.my/
language English
topic Pharmaceutical industry
Botanical chemistry. Phytochemicals
spellingShingle Pharmaceutical industry
Botanical chemistry. Phytochemicals
Abdul Bari, Dayana
Synthesis of high molecular weight DNA ladder / Dayana Abdul Bari
description DNA ladder has been used as a tool in laboratories to determine the size of DNA fragment. The aim of this study is to synthesize high molecular weight DNA ladder and to optimize PCR reaction in order to obtain high yield and specific product. High molecular weight DNA ladder ranges from 0.5 - 10 kbp. DNA ladder is synthesized by using Polymerase Chain Reaction (PCR) method, where lambda phage DNA is used as a template for both PCR reaction and designing primer. The results showed that high molecular weight DNA ladder could be synthesized in laboratory setup. Thus, it could be used as a laboratory tool in the future and may reduce the cost incurred by the faculty in carrying out related studies.
format Thesis
author Abdul Bari, Dayana
author_facet Abdul Bari, Dayana
author_sort Abdul Bari, Dayana
title Synthesis of high molecular weight DNA ladder / Dayana Abdul Bari
title_short Synthesis of high molecular weight DNA ladder / Dayana Abdul Bari
title_full Synthesis of high molecular weight DNA ladder / Dayana Abdul Bari
title_fullStr Synthesis of high molecular weight DNA ladder / Dayana Abdul Bari
title_full_unstemmed Synthesis of high molecular weight DNA ladder / Dayana Abdul Bari
title_sort synthesis of high molecular weight dna ladder / dayana abdul bari
publishDate 2009
url https://ir.uitm.edu.my/id/eprint/105530/1/105530.PDF
https://ir.uitm.edu.my/id/eprint/105530/
_version_ 1817847317960589312
score 13.222552

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