Malaysian traditional herb: CYP - herb interaction on CYP1A2 enzyme / Nurulhusna Syuhaidah Yusof

• Main Author: Yusof, Nurulhusna Syuhaidah
• Format: Thesis
• Language: English
• Published: 2009
• Subjects: Pharmaceutical industry; Pharmaceutical chemistry
• Online Access: https://ir.uitm.edu.my/id/eprint/105524/1/105524.PDF; https://ir.uitm.edu.my/id/eprint/105524/

Malaysian traditional herbs had been observed to be more popular among Malaysian populations. People belief that traditional herbs is more curative than modem medicine without notice that herbs also contains constituents that can undergo some effect on metabolizing enzyme inside human body. This experiment will evaluate the interactions of 56 species of Malaysian traditional herbs with CYP1A2 enzyme. In human liver, CYP 1 A2 enzyme consists of about 10-15% of the total CYP content of human liver. This assay had used Vivid® CYP450 Screening Kits which designed to assess metabolism and inhibition of the predominant human P450 isozymes involved in hepatic metabolism. By using this kits, interactions of 56 species of Malaysian traditional herbs, n-naphthoflavone (positive control) and dimethyl sulfoxide, DMSO (solvent control) at CYP 1 A2 enzyme were analyzed through intensity of fluorescent. Based on this study, it is showed that DMSO (solvent control) has higher fluorescent intensity. This means that it has no inhibition at CYP1A2 enzyme. A-naphthoflavone (positive control) had showed inhibition at CYP1A2 enzyme as low fluorescent intensity was observed. Meanwhile, among 56 species of these Malaysian traditional herbs, 18 samples of these herbs, including misai kucing, pegaga, pinang and tongkat ali had showed positive inhibition at CYP l A2 enzyme.