Investigation of the relationship between 5-HT2C receptor gene polymorphism with eating habit / Nur Liayana Badrol Kamar

5-HT2Creceptor plays a major role in feeding and weight gain. The polymorphism of the receptor may become a possible reason behind different level of appetite and amount of food intake in different people. Aim of this research is to explore the frequency of 5- HT zc receptor gene variant in universi...

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Main Author: Badrol Kamar, Nur Liayana
Format: Thesis
Language:English
Published: 2009
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Online Access:https://ir.uitm.edu.my/id/eprint/105411/1/105411.PDF
https://ir.uitm.edu.my/id/eprint/105411/
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spelling my.uitm.ir.1054112024-12-04T14:52:43Z https://ir.uitm.edu.my/id/eprint/105411/ Investigation of the relationship between 5-HT2C receptor gene polymorphism with eating habit / Nur Liayana Badrol Kamar Badrol Kamar, Nur Liayana Pharmaceutical industry Pharmaceutical chemistry 5-HT2Creceptor plays a major role in feeding and weight gain. The polymorphism of the receptor may become a possible reason behind different level of appetite and amount of food intake in different people. Aim of this research is to explore the frequency of 5- HT zc receptor gene variant in university student, to investigate the relationship between polymorphism of 5-HT2c receptor gene and eating habit and Body Mass Index, and to develop a PCR method that is able to detect the polymorphism of 5-HT2c receptor gene. 14 subjects were screened for polymorphisms at the promoter region of 5-HT2c (rs3813928 GIA, rs3813929 CIT, rs1414334 CIG).DNA were amplified using Polymerase Chain Reaction method. The association between BMI and 5-HT2cR genotypes was investigated with univariate analysis of variance (ANOVA) and Independent T-Test. The association between appetite and 5-HT2c receptor genotypes was investigated with Mann-Whitney U Test and Kruskal-Wallis test. As a result, it shows that genotype of rs3813939 indeed has a significant difference in the common allele and heterozygotes allele mean BMI where subject who are heterozygotes has bigger BMI compared to the common allele (p=0.012). However, the result shows that there are no significant different between level of appetite across different genotype of the subjects. Stepwise Regression analyses were done to find genotype that has the most influences on its Mean BMI and appetite. From the analysis, genotype rs3183929 has the most influences on its BMI with p=0.012 (p<0.05). However, regression analysis shows that all genotype did not significantly influence its mean appetite level. Furthermore it shows that the frequencies of allele in female university student almost follow the same frequencies of population density studied in East Asian population. As conclusion, the polymorphism of rs3183929C>T at promoter region of 5-HT2c receptor did have an influences on the BMI of the carrier, where heterozygotes are at greater risk of obesity compared to homozygote carrier. However the study did not found any association between genotype differences and the level of appetite. 2009 Thesis NonPeerReviewed text en https://ir.uitm.edu.my/id/eprint/105411/1/105411.PDF Investigation of the relationship between 5-HT2C receptor gene polymorphism with eating habit / Nur Liayana Badrol Kamar. (2009) Degree thesis, thesis, Universiti Teknologi MARA (Kampus Puncak Alam).
institution Universiti Teknologi Mara
building Tun Abdul Razak Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Teknologi Mara
content_source UiTM Institutional Repository
url_provider http://ir.uitm.edu.my/
language English
topic Pharmaceutical industry
Pharmaceutical chemistry
spellingShingle Pharmaceutical industry
Pharmaceutical chemistry
Badrol Kamar, Nur Liayana
Investigation of the relationship between 5-HT2C receptor gene polymorphism with eating habit / Nur Liayana Badrol Kamar
description 5-HT2Creceptor plays a major role in feeding and weight gain. The polymorphism of the receptor may become a possible reason behind different level of appetite and amount of food intake in different people. Aim of this research is to explore the frequency of 5- HT zc receptor gene variant in university student, to investigate the relationship between polymorphism of 5-HT2c receptor gene and eating habit and Body Mass Index, and to develop a PCR method that is able to detect the polymorphism of 5-HT2c receptor gene. 14 subjects were screened for polymorphisms at the promoter region of 5-HT2c (rs3813928 GIA, rs3813929 CIT, rs1414334 CIG).DNA were amplified using Polymerase Chain Reaction method. The association between BMI and 5-HT2cR genotypes was investigated with univariate analysis of variance (ANOVA) and Independent T-Test. The association between appetite and 5-HT2c receptor genotypes was investigated with Mann-Whitney U Test and Kruskal-Wallis test. As a result, it shows that genotype of rs3813939 indeed has a significant difference in the common allele and heterozygotes allele mean BMI where subject who are heterozygotes has bigger BMI compared to the common allele (p=0.012). However, the result shows that there are no significant different between level of appetite across different genotype of the subjects. Stepwise Regression analyses were done to find genotype that has the most influences on its Mean BMI and appetite. From the analysis, genotype rs3183929 has the most influences on its BMI with p=0.012 (p<0.05). However, regression analysis shows that all genotype did not significantly influence its mean appetite level. Furthermore it shows that the frequencies of allele in female university student almost follow the same frequencies of population density studied in East Asian population. As conclusion, the polymorphism of rs3183929C>T at promoter region of 5-HT2c receptor did have an influences on the BMI of the carrier, where heterozygotes are at greater risk of obesity compared to homozygote carrier. However the study did not found any association between genotype differences and the level of appetite.
format Thesis
author Badrol Kamar, Nur Liayana
author_facet Badrol Kamar, Nur Liayana
author_sort Badrol Kamar, Nur Liayana
title Investigation of the relationship between 5-HT2C receptor gene polymorphism with eating habit / Nur Liayana Badrol Kamar
title_short Investigation of the relationship between 5-HT2C receptor gene polymorphism with eating habit / Nur Liayana Badrol Kamar
title_full Investigation of the relationship between 5-HT2C receptor gene polymorphism with eating habit / Nur Liayana Badrol Kamar
title_fullStr Investigation of the relationship between 5-HT2C receptor gene polymorphism with eating habit / Nur Liayana Badrol Kamar
title_full_unstemmed Investigation of the relationship between 5-HT2C receptor gene polymorphism with eating habit / Nur Liayana Badrol Kamar
title_sort investigation of the relationship between 5-ht2c receptor gene polymorphism with eating habit / nur liayana badrol kamar
publishDate 2009
url https://ir.uitm.edu.my/id/eprint/105411/1/105411.PDF
https://ir.uitm.edu.my/id/eprint/105411/
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score 13.222552