Investigation of the factors that influence long range PCR / Muhamad Saifuzzaman Mohamad Kassim

• Main Author: Mohamad Kassim, Muhamad Saifuzzaman
• Format: Thesis
• Language: English
• Published: 2009
• Subjects: Pharmaceutical industry; Pharmaceutical chemistry
• Online Access: https://ir.uitm.edu.my/id/eprint/105247/1/105247.pdf; https://ir.uitm.edu.my/id/eprint/105247/

Long range PCR method for uniform amplification of specific lambda DNA sequence is described. The careful design of all 6 pairs of primers and optimum temperature cycling profile produce good result for long range PCR. Three pairs of 15kb and three pairs of 20kb primers were design using oligo explo...