Dose-response effects on spatial learning and memory after subacute exposure of HAB10R12 endophytic extract in mice / Radhia Azia Redzuan

• Main Author: Redzuan, Radhia Azia
• Format: Thesis
• Language: English
• Published: 2008
• Subjects: Pharmaceutical dosage forms
• Online Access: https://ir.uitm.edu.my/id/eprint/101224/1/101224.PDF; https://ir.uitm.edu.my/id/eprint/101224/

An endophyte is an endosymbiont, often a bacterium or fungus, which lives within a plant for at least part of its life without causing apparent disease. Endophytes may benefit host plants by preventing pathogenic organisms from colonizing them. Our strain of interest is HAB10R12 , which is found in local forest. Preliminary studies have shown that extract from this strain might have some neuroprotective properties. This study was aimed to investigate the dose-response effects of endophytic extract of HAB10R12 on spatial learning and memory after subacute exposure in mice. For the evaluation of the dose-response effects on spatial learning and memory, the Morris Water Maze (MWM) test is performed. The endophytic extract dose of 5, 10, 20 mg/kg were subacutely administered through the intraperitoneal route to mice (n = 6 per group) everyday for 14 days. Normal saline was administered intraperitoneally as a control and memantine (an NMDA receptor antagonist) as a positive control. The Morris Water Maze test was carried out on day 3, 6, 9, 12, 15 (after 15 minutes of treatment). From the result obtained only memantine showed a significant difference in enhancing the spatial learning and memory compared to the blank control. The 5mg/kg, 10 mg/kg of the HAB10R12 endophytic extract did not show statistically significant difference (P > 0.05) towards the spatial learning and memory. Further studies should be conducted to uncover the possible action of HAB10R12 endophytic extract on the human pathophysiology