Molecular analysis of weak d among non-chinese blood donors using specific sequence primer- polymerase chain reaction (SSP-PCR) / Mohd Sodiq Rifaei Hilmi

• Main Author: Hilmi, Mohd Sodiq Rifaei
• Format: Thesis
• Language: English
• Published: 2016
• Online Access: https://ir.uitm.edu.my/id/eprint/101207/1/101207.pdf; https://ir.uitm.edu.my/id/eprint/101207/

Determination of weak D types may reduce the risk of unnecessary alloimmunization and also beneficial for proper utilization of Rh negative blood units. Present study aimed to determine the weak D type among non-Chinese blood donors from National Blood Centre (NBC) Kuala Lumpur by using Polymerase chain reaction with specific sequence primer (PCR-SSP). Out of 104 Rh negative blood donors, only one turn out to be positive for weak D, but undefined to be detected by selected kits. The phenotype for the India sample were dCce. Rh phenotyping of all sample consist of 96% dce, 2.9% dCce, and 0.9% dCcEe. In conclusion, due to time constrained for sample collection and the use of kits, the weak D type undetermined. Hence, it is recommended to prolong the duration of the samples collection and to use the different weak D variant kits or using real time PCR.