Evaluation of Antioxidant and Antimicrobial Activity of Saponin Extracts from Different Parts of Argania spinosa L. Skeels
The argan tree is a versatile forest tree (silviculture-fruit-forestry) of great importance for the country both in biological, phytogenetic and ecological biodiversity as well as in economic and social aspects. It has significant medicinal and therapeutic potential. The present study concentrated o...
Saved in:
Main Authors: | , , , , , , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
HH Publisher
2023
|
Subjects: | |
Online Access: | http://eprints.sunway.edu.my/2900/1/Long%20Chiau%20Ming_Evaluation%20of%20antioxidant%20and%20antimicrobial%20activity%20of%20saponin%20extracts.pdf http://eprints.sunway.edu.my/2900/ https://doi.org/10.36877/pmmb.a0000338 |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
id |
my.sunway.eprints.2900 |
---|---|
record_format |
eprints |
spelling |
my.sunway.eprints.29002024-07-26T01:36:23Z http://eprints.sunway.edu.my/2900/ Evaluation of Antioxidant and Antimicrobial Activity of Saponin Extracts from Different Parts of Argania spinosa L. Skeels El Idrissi, Yousra Elouafy, Youssef El Moudden, Hamza Mghazli, Najoua El Guezzane, Chakir El Yadini, Adil Harhar, Hicham Zarrouk, Abdelkader Goh, Khang Wen Long, Chiau Ming * Bouyahya, Abdelhakim Tabyaoui, Mohamed QK Botany RM Therapeutics. Pharmacology RS Pharmacy and materia medica TP Chemical technology The argan tree is a versatile forest tree (silviculture-fruit-forestry) of great importance for the country both in biological, phytogenetic and ecological biodiversity as well as in economic and social aspects. It has significant medicinal and therapeutic potential. The present study concentrated on saccharide-containing molecules, particularly saponins, for their medicinal properties. Butanol was used to fractionate phytochemical groups from different parts of Argania spinosa (branch, leaf, pulp, shell and seed), which were subsequently precipitated in ether. It was found that solvent fractionation increased the total saponin amount according to the colorimetric test using vanillin and sulfuric acid. The butanol fraction of the argan shell and its precipitate showed the highest levels of triterpene saponins compared to the rest of the plant parts studied. The samples showed antioxidant capacity by DPPH versus ascorbic acid (IC50 = 1.92 µg/mL) and by ABTS using the similar standard (IC50 = 11.31 µg/mL). The antioxidant activity of the samples was significantly improved from the crude shell extract (IC50 = 5.85 µg/mL) to its butanolic fraction (IC50 = 2.94 µg/mL) and its precipitate (IC50 = 1.57 µg/mL) using 2,2-diphenyl-picrylhydrazyl. Indeed, the ABTS test, showed similarity to the core results. Also, the solid disc diffusion method was used to highlight the antimicrobial activity of extracts from different parts of the Argania spinosa plant. The EtOH 70% and Aqueous leaf extracts exhibited the highest antibacterial activity, resulting in an inhibition zone of approximately 13 mm against the growth of Staphylococcus saprophyticus. On the other hand, the extract of saponin filtrate from the pulp part of the plant displayed the lowest antibacterial activity, with an inhibition zone of 9 mm. All studied types of saponin extract from different parts of the plant do not contribute to any antifungal activity against Botrytis cinerea. HH Publisher 2023 Article PeerReviewed text en cc_by_nc_4 http://eprints.sunway.edu.my/2900/1/Long%20Chiau%20Ming_Evaluation%20of%20antioxidant%20and%20antimicrobial%20activity%20of%20saponin%20extracts.pdf El Idrissi, Yousra and Elouafy, Youssef and El Moudden, Hamza and Mghazli, Najoua and El Guezzane, Chakir and El Yadini, Adil and Harhar, Hicham and Zarrouk, Abdelkader and Goh, Khang Wen and Long, Chiau Ming * and Bouyahya, Abdelhakim and Tabyaoui, Mohamed (2023) Evaluation of Antioxidant and Antimicrobial Activity of Saponin Extracts from Different Parts of Argania spinosa L. Skeels. Progress in Microbes & Molecular Biology, 6 (1). ISSN 2637-1049 https://doi.org/10.36877/pmmb.a0000338 10.36877/pmmb.a0000338 |
institution |
Sunway University |
building |
Sunway Campus Library |
collection |
Institutional Repository |
continent |
Asia |
country |
Malaysia |
content_provider |
Sunway University |
content_source |
Sunway Institutional Repository |
url_provider |
http://eprints.sunway.edu.my/ |
language |
English |
topic |
QK Botany RM Therapeutics. Pharmacology RS Pharmacy and materia medica TP Chemical technology |
spellingShingle |
QK Botany RM Therapeutics. Pharmacology RS Pharmacy and materia medica TP Chemical technology El Idrissi, Yousra Elouafy, Youssef El Moudden, Hamza Mghazli, Najoua El Guezzane, Chakir El Yadini, Adil Harhar, Hicham Zarrouk, Abdelkader Goh, Khang Wen Long, Chiau Ming * Bouyahya, Abdelhakim Tabyaoui, Mohamed Evaluation of Antioxidant and Antimicrobial Activity of Saponin Extracts from Different Parts of Argania spinosa L. Skeels |
description |
The argan tree is a versatile forest tree (silviculture-fruit-forestry) of great importance for the country both in biological, phytogenetic and ecological biodiversity as well as in economic and social aspects. It has significant medicinal and therapeutic potential. The present study concentrated on saccharide-containing molecules, particularly saponins, for their medicinal properties. Butanol was used to fractionate phytochemical groups from different parts of Argania spinosa (branch, leaf, pulp, shell and seed), which were subsequently precipitated in ether. It was found that solvent fractionation increased the total saponin amount according to the colorimetric test using vanillin and sulfuric acid. The butanol fraction of the argan shell and its precipitate showed the highest levels of triterpene saponins compared to the rest of the plant parts studied. The samples showed antioxidant capacity by DPPH versus ascorbic acid (IC50 = 1.92 µg/mL) and by ABTS using the similar standard (IC50 = 11.31 µg/mL). The antioxidant activity of the samples was significantly improved from the crude shell extract (IC50 = 5.85 µg/mL) to its butanolic fraction (IC50 = 2.94 µg/mL) and its precipitate (IC50 = 1.57 µg/mL) using 2,2-diphenyl-picrylhydrazyl. Indeed, the ABTS test, showed similarity to the core results. Also, the solid disc diffusion method was used to highlight the antimicrobial activity of extracts from different parts of the Argania spinosa plant. The EtOH 70% and Aqueous leaf extracts exhibited the highest antibacterial activity, resulting in an inhibition zone of approximately 13 mm against the growth of Staphylococcus saprophyticus. On the other hand, the extract of saponin filtrate from the pulp part of the plant displayed the lowest antibacterial activity, with an inhibition zone of 9 mm. All studied types of saponin extract from different parts of the plant do not contribute to any antifungal activity against Botrytis cinerea. |
format |
Article |
author |
El Idrissi, Yousra Elouafy, Youssef El Moudden, Hamza Mghazli, Najoua El Guezzane, Chakir El Yadini, Adil Harhar, Hicham Zarrouk, Abdelkader Goh, Khang Wen Long, Chiau Ming * Bouyahya, Abdelhakim Tabyaoui, Mohamed |
author_facet |
El Idrissi, Yousra Elouafy, Youssef El Moudden, Hamza Mghazli, Najoua El Guezzane, Chakir El Yadini, Adil Harhar, Hicham Zarrouk, Abdelkader Goh, Khang Wen Long, Chiau Ming * Bouyahya, Abdelhakim Tabyaoui, Mohamed |
author_sort |
El Idrissi, Yousra |
title |
Evaluation of Antioxidant and Antimicrobial Activity of Saponin Extracts from Different Parts of Argania spinosa L. Skeels |
title_short |
Evaluation of Antioxidant and Antimicrobial Activity of Saponin Extracts from Different Parts of Argania spinosa L. Skeels |
title_full |
Evaluation of Antioxidant and Antimicrobial Activity of Saponin Extracts from Different Parts of Argania spinosa L. Skeels |
title_fullStr |
Evaluation of Antioxidant and Antimicrobial Activity of Saponin Extracts from Different Parts of Argania spinosa L. Skeels |
title_full_unstemmed |
Evaluation of Antioxidant and Antimicrobial Activity of Saponin Extracts from Different Parts of Argania spinosa L. Skeels |
title_sort |
evaluation of antioxidant and antimicrobial activity of saponin extracts from different parts of argania spinosa l. skeels |
publisher |
HH Publisher |
publishDate |
2023 |
url |
http://eprints.sunway.edu.my/2900/1/Long%20Chiau%20Ming_Evaluation%20of%20antioxidant%20and%20antimicrobial%20activity%20of%20saponin%20extracts.pdf http://eprints.sunway.edu.my/2900/ https://doi.org/10.36877/pmmb.a0000338 |
_version_ |
1805893836195495936 |
score |
13.18916 |