Enhanced antibacterial and anticancer activities of plant extract mediated green synthesized zinc oxide-silver nanoparticles
This study presents a green synthesis approach for the fabrication of zinc oxide-silver nanoparticles (ZnO-Ag-NPs) using Punica granatum fruit peels extract as a natural reducing and stabilizing agent. This eco-friendly method offers a sustainable alternative to conventional methods that often emplo...
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my.sunway.eprints.27652024-07-09T00:24:44Z http://eprints.sunway.edu.my/2765/ Enhanced antibacterial and anticancer activities of plant extract mediated green synthesized zinc oxide-silver nanoparticles Siti Nur Amalina, Mohamad Sukri Shameli, Kamyar Teow, Sin Yeang * Chew, Jactty * Ooi, Li Ting Soon, Michiele Lee Kiun * Nur Afini, Ismail Moeini, Hassan QK Botany QR Microbiology RM Therapeutics. Pharmacology RS Pharmacy and materia medica This study presents a green synthesis approach for the fabrication of zinc oxide-silver nanoparticles (ZnO-Ag-NPs) using Punica granatum fruit peels extract as a natural reducing and stabilizing agent. This eco-friendly method offers a sustainable alternative to conventional methods that often employ toxic or hazardous chemicals. Antibacterial and anti-cancer activities of the green synthesized nanoparticles were then assessed in vitro. X-ray diffraction confirmed the production of ZnO-Ag-NPs with increasing crystallinity in higher pH values. The ZnO-Ag-NPs were found to be agglomerated with spherical Ag-NPs. Fourier Transform Infrared (FTIR) spectra revealed a broad band in ZnO-Ag-NPs ranging from 400-1 to 530 cm-1 with reduced intensity as compared to ZnO-NPs, indicating the formation of Ag-NPs on the surface of ZnO-NPs. The synthesized ZnO-Ag-NPs exhibited potent antibacterial activity against a broad spectrum of bacterial strains, particularly Gram-positive bacteria, with superior inhibition activity compared to ZnO-NPs. Moreover, ZnO-Ag-NPs showed a dose-dependent anti-proliferative effect on colorectal-, lung-, and cervical cancer cells. ZnO-Ag-NPs showed significantly greater efficacy in inhibiting cancer cell growth at a lower concentration of 31.25 μg/mL, compared to ZnO-NPs which required over 500 μg/mL, possibly due to the presence of silver nanoparticles (Ag-NPs). The results obtained from this study demonstrate the potential of green synthesis approaches in the fabrication of therapeutic nanomaterials for cancer treatment, as well as other biomedical applications. Frontiers Media 2023 Article PeerReviewed Siti Nur Amalina, Mohamad Sukri and Shameli, Kamyar and Teow, Sin Yeang * and Chew, Jactty * and Ooi, Li Ting and Soon, Michiele Lee Kiun * and Nur Afini, Ismail and Moeini, Hassan (2023) Enhanced antibacterial and anticancer activities of plant extract mediated green synthesized zinc oxide-silver nanoparticles. Frontiers in Microbiology, 14. ISSN 1664-302X https://doi.org/10.3389/fmicb.2023.1194292 10.3389/fmicb.2023.1194292 |
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QK Botany QR Microbiology RM Therapeutics. Pharmacology RS Pharmacy and materia medica Siti Nur Amalina, Mohamad Sukri Shameli, Kamyar Teow, Sin Yeang * Chew, Jactty * Ooi, Li Ting Soon, Michiele Lee Kiun * Nur Afini, Ismail Moeini, Hassan Enhanced antibacterial and anticancer activities of plant extract mediated green synthesized zinc oxide-silver nanoparticles |
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This study presents a green synthesis approach for the fabrication of zinc oxide-silver nanoparticles (ZnO-Ag-NPs) using Punica granatum fruit peels extract as a natural reducing and stabilizing agent. This eco-friendly method offers a sustainable alternative to conventional methods that often employ toxic or hazardous chemicals. Antibacterial and anti-cancer activities of the green synthesized nanoparticles were then assessed in vitro. X-ray diffraction confirmed the production of ZnO-Ag-NPs with increasing crystallinity in higher pH values. The ZnO-Ag-NPs were found to be agglomerated with spherical Ag-NPs. Fourier Transform Infrared (FTIR) spectra revealed a broad band in ZnO-Ag-NPs ranging from 400-1 to 530 cm-1 with reduced intensity as compared to ZnO-NPs, indicating the formation of Ag-NPs on the surface of ZnO-NPs. The synthesized ZnO-Ag-NPs exhibited potent antibacterial activity against a broad spectrum of bacterial strains, particularly Gram-positive bacteria, with superior inhibition activity compared to ZnO-NPs. Moreover, ZnO-Ag-NPs showed a dose-dependent anti-proliferative effect on colorectal-, lung-, and cervical cancer cells. ZnO-Ag-NPs showed significantly greater efficacy in inhibiting cancer cell growth at a lower concentration of 31.25 μg/mL, compared to ZnO-NPs which required over 500 μg/mL, possibly due to the presence of silver nanoparticles (Ag-NPs). The results obtained from this study demonstrate the potential of green synthesis approaches in the fabrication of therapeutic nanomaterials for cancer treatment, as well as other biomedical applications. |
format |
Article |
author |
Siti Nur Amalina, Mohamad Sukri Shameli, Kamyar Teow, Sin Yeang * Chew, Jactty * Ooi, Li Ting Soon, Michiele Lee Kiun * Nur Afini, Ismail Moeini, Hassan |
author_facet |
Siti Nur Amalina, Mohamad Sukri Shameli, Kamyar Teow, Sin Yeang * Chew, Jactty * Ooi, Li Ting Soon, Michiele Lee Kiun * Nur Afini, Ismail Moeini, Hassan |
author_sort |
Siti Nur Amalina, Mohamad Sukri |
title |
Enhanced antibacterial and anticancer activities of plant extract mediated green synthesized zinc oxide-silver nanoparticles |
title_short |
Enhanced antibacterial and anticancer activities of plant extract mediated green synthesized zinc oxide-silver nanoparticles |
title_full |
Enhanced antibacterial and anticancer activities of plant extract mediated green synthesized zinc oxide-silver nanoparticles |
title_fullStr |
Enhanced antibacterial and anticancer activities of plant extract mediated green synthesized zinc oxide-silver nanoparticles |
title_full_unstemmed |
Enhanced antibacterial and anticancer activities of plant extract mediated green synthesized zinc oxide-silver nanoparticles |
title_sort |
enhanced antibacterial and anticancer activities of plant extract mediated green synthesized zinc oxide-silver nanoparticles |
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Frontiers Media |
publishDate |
2023 |
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http://eprints.sunway.edu.my/2765/ https://doi.org/10.3389/fmicb.2023.1194292 |
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1804069669367185408 |
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