The effect of prolonged fixation time on haematoxylin & eosin staining quality of rats colon and placenta tissue

• Main Authors: Mohd Radzuan, Hazulin, Wan Omar, Wan Fatein Nabeila, Saharudin, Shahida, Abd Rahim, Nour El Huda, Halim, Mohd Dhiyaulhaq
• Format: Article
• Language: English
• Published: IIUM Press 2022
• Subjects: Q Science (General); QM Human anatomy; R Medicine (General); RZ Other systems of medicine
• Online Access: http://irep.iium.edu.my/99857/3/99857_The%20effect%20of%20prolonged%20fixation%20time%20on%20haematoxylin.pdf; http://irep.iium.edu.my/99857/; https://journals.iium.edu.my/ktn/index.php/ijohs/issue/view/9/8

The use of formalin in histopathological sample preparation is intended to preserve protein and cellular organelles1. It is argued that prolonged formalin fixation may lead to tissue shrinkage and hardening2,3. Reports on immunohistochemical studies showed that this does not cause reduction in antig...