Cloning, expression, purification, crystallization and preliminary X-ray studies of the C-terminal domain of Rv3262 (FbiB) from Mycobacterium tuberculosis

• Main Authors: Mohamed Rehan, Aisyah, Paterson, Neil G., Baker, Edward N., Squire, Christopher J.
• 格式: Article
• 语言: English
• 出版: Wiley-Blackwell Publishing, Inc. 2011
• 主题: Q Science (General); QH301 Biology
• 在线阅读: http://irep.iium.edu.my/9893/4/CLONING%2CEXPRESSION%2C_PURIFICATION%2C_CRYSTALLIZATION_AND_PRELIMINARY_X-RAY_STUDIES_OF_THE_C-TERMINAL.pdf; http://irep.iium.edu.my/9893/; http://scripts.iucr.org/cgi-bin/paper?S1744309111028958

During cofactor F420 biosynthesis, the enzyme F420-[gamma]-glutamyl ligase (FbiB) catalyzes the addition of [gamma]-linked L-glutamate residues to form polyglutamylated F420 derivatives. In Mycobacterium tuberculosis, Rv3262 (FbiB) consists of two domains: an N-terminal domain from the F420 ligase s...