Optimum Medium Components for Biosurfactant Production by Klebsiella pneumoniae WMF02 Utilizing Sludge Palm Oil as a Substrate

• Main Authors: Jamal, Parveen, Wan Nawawi, Wan Mohd Fazli, Alam, Md. Zahangir
• Format: Conference or Workshop Item
• Language: English
• Published: 2011
• Subjects: TA164 Bioengineering; TA170 Environmental engineering; TP248.13 Biotechnology
• Online Access: http://irep.iium.edu.my/9758/1/wan.pdf; http://irep.iium.edu.my/9758/; http://www.iium.edu.my/icbioe/2011/

In the present study, optimizing critical nutritional constituents was attempted as a primary strategy to improve biosurfactant production from Klebsiella pneumoniae WMF02 in liquid state fermentation utilizing sludge palm oil as a substrate. One-factor-at-a-time (OFAT) optimization was employed to evaluate the effects of sludge palm oil (SPO), sucrose, MgSO4, FeSO4, NaNO3 and K2HPO4 on surface tension reductivity. Sucrose was selected as a co-substrate over glucose in production media. The optimal levels of the aforementioned variables were (g/l) sucrose 5.0, MgSO4 0.4, FeSO4 0.3, NaNO3 2.0, and K2HPO4 4.0, with SPO concentration of 4% (v/v). The optimized medium shows surface tension reduction from 36.2 mN/m (non-optimized medium) to 25.70 mN/m. Preliminary biosurfactant identification indicated that the biosurfactant produced was phospholipids in nature.