The effect of non-polar solvent extraction of Piper Ribesioides (Sireh Hutan) on Candida Albicans Morphology
Introduction: Piper ribesioides is a vigorous climber, found in Indonesia and the Peninsular Malaysia, the root of this piper is used to treat an illness caused from asthma, diarrhoea and abdominal pain. Stems of P. ribesioides have long been used to flavour food giving its spicy and peppery t...
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my.iium.irep.941842021-11-29T09:14:30Z http://irep.iium.edu.my/94184/ The effect of non-polar solvent extraction of Piper Ribesioides (Sireh Hutan) on Candida Albicans Morphology P.M, Ridzuan S.V, Sathiya Draman, Samsul Maizatu-Syamemy, M.N. Masharudin, A.W. Amilin, A.N. Farihah, S.N. Badhrulhisham, Aisyah Naimat, Faiza R Medicine (General) RA Public aspects of medicine RM Therapeutics. Pharmacology RS Pharmacy and materia medica Introduction: Piper ribesioides is a vigorous climber, found in Indonesia and the Peninsular Malaysia, the root of this piper is used to treat an illness caused from asthma, diarrhoea and abdominal pain. Stems of P. ribesioides have long been used to flavour food giving its spicy and peppery taste. Candida albicans is a commensal life form in the human cutaneous and mucosal verdure, however can turn into a noteworthy human contagious pathogen given the best possible conditions considering hyphal separation, biofilm arrangement, and excess. This fungus is also responsible in causing sexually transmitted infection. The most basic regions for candidiasis are the oral and gastrointestinal tract, the vaginal and the skin. The aim of this study is to identify the potential activities of non-polar extraction of P. ribesiodes on C. albicans morphology. Methods: P. ribesiodes was extracted using n-hexane solvent and serial dilution method was used to produce concentration from 500, 250, 125, 62.5, 31.3, 15.63, 7.81, 3.91, 1.96 and 0.98 mg/ml. Sensitivity test of C. albicans was measured through the diameter of inhibition zone when treated with P. ribesiodes. Minimum Inhibitory Concentration (MIC) and Minimum Fungicidal Concentrations (MFC) were determined by using broth micro-diluted method. Morphology study was done by using Scanning Electron Microscope (SEM). Results: The largest zone of inhibition in 24 hours was at 500 mg/ml concentration. The diameter was nearly 10.333 ± 2.309 mm. The Minimum Inhibitory Concentration and Minimum Fungal Concentration were 15.63 mg/ml and 31.25 mg/ml respectively. Morphology study revealed erosion and damage of cell wall of C. albicans. Conclusion: Morphology of C. albicans were altered when treated with n-hexane extraction of P. ribesiodes. Universiti Kuala Lumpur 2021-11 Article PeerReviewed application/pdf en http://irep.iium.edu.my/94184/1/94184_The%20effect%20of%20non-polar%20solvent%20extraction.pdf P.M, Ridzuan and S.V, Sathiya and Draman, Samsul and Maizatu-Syamemy, M.N. and Masharudin, A.W. and Amilin, A.N. and Farihah, S.N. and Badhrulhisham, Aisyah and Naimat, Faiza (2021) The effect of non-polar solvent extraction of Piper Ribesioides (Sireh Hutan) on Candida Albicans Morphology. Asian Journal of Medicine and Health Sciences, 4 (2). pp. 37-46. E-ISSN 2637-0603 https://www.ajmhsrcmp.org/images/journal/Vol4_Issue2_Nov21/06_Ridzuan_AJMHS_2021_Vol4_Issue2_OriginalArticle_Candida.pdf |
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R Medicine (General) RA Public aspects of medicine RM Therapeutics. Pharmacology RS Pharmacy and materia medica P.M, Ridzuan S.V, Sathiya Draman, Samsul Maizatu-Syamemy, M.N. Masharudin, A.W. Amilin, A.N. Farihah, S.N. Badhrulhisham, Aisyah Naimat, Faiza The effect of non-polar solvent extraction of Piper Ribesioides (Sireh Hutan) on Candida Albicans Morphology |
description |
Introduction: Piper ribesioides is a vigorous climber, found in Indonesia and the Peninsular
Malaysia, the root of this piper is used to treat an illness caused from asthma, diarrhoea and
abdominal pain. Stems of P. ribesioides have long been used to flavour food giving its spicy and
peppery taste. Candida albicans is a commensal life form in the human cutaneous and mucosal
verdure, however can turn into a noteworthy human contagious pathogen given the best possible
conditions considering hyphal separation, biofilm arrangement, and excess. This fungus is also
responsible in causing sexually transmitted infection. The most basic regions for candidiasis are
the oral and gastrointestinal tract, the vaginal and the skin. The aim of this study is to identify the
potential activities of non-polar extraction of P. ribesiodes on C. albicans morphology.
Methods: P. ribesiodes was extracted using n-hexane solvent and serial dilution method was used
to produce concentration from 500, 250, 125, 62.5, 31.3, 15.63, 7.81, 3.91, 1.96 and 0.98 mg/ml.
Sensitivity test of C. albicans was measured through the diameter of inhibition zone when treated
with P. ribesiodes. Minimum Inhibitory Concentration (MIC) and Minimum Fungicidal
Concentrations (MFC) were determined by using broth micro-diluted method. Morphology study
was done by using Scanning Electron Microscope (SEM).
Results: The largest zone of inhibition in 24 hours was at 500 mg/ml concentration. The diameter
was nearly 10.333 ± 2.309 mm. The Minimum Inhibitory Concentration and Minimum Fungal
Concentration were 15.63 mg/ml and 31.25 mg/ml respectively. Morphology study revealed
erosion and damage of cell wall of C. albicans.
Conclusion: Morphology of C. albicans were altered when treated with n-hexane extraction of
P. ribesiodes. |
format |
Article |
author |
P.M, Ridzuan S.V, Sathiya Draman, Samsul Maizatu-Syamemy, M.N. Masharudin, A.W. Amilin, A.N. Farihah, S.N. Badhrulhisham, Aisyah Naimat, Faiza |
author_facet |
P.M, Ridzuan S.V, Sathiya Draman, Samsul Maizatu-Syamemy, M.N. Masharudin, A.W. Amilin, A.N. Farihah, S.N. Badhrulhisham, Aisyah Naimat, Faiza |
author_sort |
P.M, Ridzuan |
title |
The effect of non-polar solvent extraction of Piper Ribesioides (Sireh Hutan) on Candida Albicans Morphology |
title_short |
The effect of non-polar solvent extraction of Piper Ribesioides (Sireh Hutan) on Candida Albicans Morphology |
title_full |
The effect of non-polar solvent extraction of Piper Ribesioides (Sireh Hutan) on Candida Albicans Morphology |
title_fullStr |
The effect of non-polar solvent extraction of Piper Ribesioides (Sireh Hutan) on Candida Albicans Morphology |
title_full_unstemmed |
The effect of non-polar solvent extraction of Piper Ribesioides (Sireh Hutan) on Candida Albicans Morphology |
title_sort |
effect of non-polar solvent extraction of piper ribesioides (sireh hutan) on candida albicans morphology |
publisher |
Universiti Kuala Lumpur |
publishDate |
2021 |
url |
http://irep.iium.edu.my/94184/1/94184_The%20effect%20of%20non-polar%20solvent%20extraction.pdf http://irep.iium.edu.my/94184/ https://www.ajmhsrcmp.org/images/journal/Vol4_Issue2_Nov21/06_Ridzuan_AJMHS_2021_Vol4_Issue2_OriginalArticle_Candida.pdf |
_version_ |
1718925629951311872 |
score |
13.160551 |