The protective effect of sodium nitroprusside(SNP)treatment on Arabidopsis thaliana seedlings exposed to toxic level of Pb is not linked to avoidance of Pb uptake
The effects of 100 μM Pb(NO3)2 on 7-day-old Arabidopsis thaliana seedlings grown from seeds pretreated with a nitric oxide donor, sodium nitroprusside (SNP), or a nitric oxide scavenger, 2-(4-carboxyphenyl)-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide (cPTIO) were investigated. Physiological stress...
Saved in:
Main Authors: | , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Academic Press
2011
|
Subjects: | |
Online Access: | http://irep.iium.edu.my/938/2/Phang_et_al_2011.PDF http://irep.iium.edu.my/938/ http://dx.doi.org/10.1016/j.ecoenv.2011.02.006 |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
id |
my.iium.irep.938 |
---|---|
record_format |
dspace |
spelling |
my.iium.irep.9382013-06-21T03:40:57Z http://irep.iium.edu.my/938/ The protective effect of sodium nitroprusside(SNP)treatment on Arabidopsis thaliana seedlings exposed to toxic level of Pb is not linked to avoidance of Pb uptake Phang, Ing Chia Leung, David W. M. Taylor, H. Harry Burritt, David J. Q Science (General) The effects of 100 μM Pb(NO3)2 on 7-day-old Arabidopsis thaliana seedlings grown from seeds pretreated with a nitric oxide donor, sodium nitroprusside (SNP), or a nitric oxide scavenger, 2-(4-carboxyphenyl)-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide (cPTIO) were investigated. Physiological stress induced by Pb (reduced root growth) was less evident in seedlings grown from seeds pretreated with SNP. However, SNP pretreatment of seeds did not affect Pb accumulation in the seedlings. Pb exposure caused oxidative stress by elevating hydrogen peroxide (H2O2) and lipid hydroperoxide contents of the seedlings. SNP pretreatment of seeds counteracted Pb toxicity by reducing the H2O2 and lipid hydroperoxide contents of Pb-exposed seedlings. Additionally, Pb-induced rises in antioxidative enzyme activities were reversed by SNP pretreatment of seeds. It was concluded that the ameliorating effects of SNP pretreatment were associated with the release of nitric oxide because cPTIO reversed these effects of SNP pretreatment of seeds and more importantly SNP pretreatment did not trigger an avoidance mechanism. Academic Press 2011-07 Article REM application/pdf en http://irep.iium.edu.my/938/2/Phang_et_al_2011.PDF Phang, Ing Chia and Leung, David W. M. and Taylor, H. Harry and Burritt, David J. (2011) The protective effect of sodium nitroprusside(SNP)treatment on Arabidopsis thaliana seedlings exposed to toxic level of Pb is not linked to avoidance of Pb uptake. Ecotoxicology and Environmental Safety, 74 (5). pp. 1310-1315. ISSN 0147-6513 http://dx.doi.org/10.1016/j.ecoenv.2011.02.006 doi:10.1016/j.ecoenv.2011.02.006 |
institution |
Universiti Islam Antarabangsa Malaysia |
building |
IIUM Library |
collection |
Institutional Repository |
continent |
Asia |
country |
Malaysia |
content_provider |
International Islamic University Malaysia |
content_source |
IIUM Repository (IREP) |
url_provider |
http://irep.iium.edu.my/ |
language |
English |
topic |
Q Science (General) |
spellingShingle |
Q Science (General) Phang, Ing Chia Leung, David W. M. Taylor, H. Harry Burritt, David J. The protective effect of sodium nitroprusside(SNP)treatment on Arabidopsis thaliana seedlings exposed to toxic level of Pb is not linked to avoidance of Pb uptake |
description |
The effects of 100 μM Pb(NO3)2 on 7-day-old Arabidopsis thaliana seedlings grown from seeds pretreated with a nitric oxide donor, sodium nitroprusside (SNP), or a nitric oxide scavenger, 2-(4-carboxyphenyl)-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide (cPTIO) were investigated. Physiological stress induced by Pb (reduced root growth) was less evident in seedlings grown from seeds pretreated with SNP. However, SNP pretreatment of seeds did not affect Pb accumulation in the seedlings. Pb exposure caused oxidative stress by elevating hydrogen peroxide (H2O2) and lipid hydroperoxide contents of the seedlings. SNP pretreatment of seeds counteracted Pb toxicity by reducing the H2O2 and lipid hydroperoxide contents of Pb-exposed seedlings. Additionally, Pb-induced rises in antioxidative enzyme activities were reversed by SNP pretreatment of seeds. It was concluded that the ameliorating effects of SNP pretreatment were associated with the release of nitric oxide because cPTIO reversed these effects of SNP pretreatment of seeds and more importantly SNP pretreatment did not trigger an avoidance mechanism. |
format |
Article |
author |
Phang, Ing Chia Leung, David W. M. Taylor, H. Harry Burritt, David J. |
author_facet |
Phang, Ing Chia Leung, David W. M. Taylor, H. Harry Burritt, David J. |
author_sort |
Phang, Ing Chia |
title |
The protective effect of sodium nitroprusside(SNP)treatment on Arabidopsis thaliana seedlings exposed to toxic level of Pb is not linked to avoidance of Pb uptake |
title_short |
The protective effect of sodium nitroprusside(SNP)treatment on Arabidopsis thaliana seedlings exposed to toxic level of Pb is not linked to avoidance of Pb uptake |
title_full |
The protective effect of sodium nitroprusside(SNP)treatment on Arabidopsis thaliana seedlings exposed to toxic level of Pb is not linked to avoidance of Pb uptake |
title_fullStr |
The protective effect of sodium nitroprusside(SNP)treatment on Arabidopsis thaliana seedlings exposed to toxic level of Pb is not linked to avoidance of Pb uptake |
title_full_unstemmed |
The protective effect of sodium nitroprusside(SNP)treatment on Arabidopsis thaliana seedlings exposed to toxic level of Pb is not linked to avoidance of Pb uptake |
title_sort |
protective effect of sodium nitroprusside(snp)treatment on arabidopsis thaliana seedlings exposed to toxic level of pb is not linked to avoidance of pb uptake |
publisher |
Academic Press |
publishDate |
2011 |
url |
http://irep.iium.edu.my/938/2/Phang_et_al_2011.PDF http://irep.iium.edu.my/938/ http://dx.doi.org/10.1016/j.ecoenv.2011.02.006 |
_version_ |
1643604696088182784 |
score |
13.18916 |