Angiotensin-converting enzyme inhibition activity and LCMS analysis of Syzygium polyanthum (Wight) Walp leaves
Syzygium polyanthum is an ethnomedicinal plant that is widely used among the Malay community for treating hypertension. Previous studies have shown that the plant leaves have a significant antihypertensive effect in rats; however, its mechanism of action and the bioactive compounds is not yet well-...
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my.iium.irep.929742021-10-26T08:02:04Z http://irep.iium.edu.my/92974/ Angiotensin-converting enzyme inhibition activity and LCMS analysis of Syzygium polyanthum (Wight) Walp leaves Tuan Anuar, Tuan Ashraf Faiz Ismail, Azlini Omar, Muhammad Nor Mohamed Suffian, Izzat Fahimuddin Abdul Hamid, Azzmer Azzar Q Science (General) R Medicine (General) RM Therapeutics. Pharmacology RM300 Drugs and their action Syzygium polyanthum is an ethnomedicinal plant that is widely used among the Malay community for treating hypertension. Previous studies have shown that the plant leaves have a significant antihypertensive effect in rats; however, its mechanism of action and the bioactive compounds is not yet well-studied. One of the possible mechanisms to reduce blood pressure is through inhibiting the angiotensin-converting enzyme (ACE). Therefore, this study aims to determine the ACE inhibitory activity of S. polyanthum leaves and its phytochemical composition. S. polyanthum leaves were dried, ground, macerated with water in a bath-sonicator, and then freeze-dried to produce the aqueous S. polyanthum (ASP) leaves extract. The ACE inhibition activity of ASP was tested using Cushman and Cheung method at a range of concentrations from 1 to 1000 µg/ml. The phytochemical composition in ASP was then analysed using Liquid Chromatography Quadrupole Time-of-Flight Mass Spectrometry (LCQTOF/MS). It was found that the concentration of ASP that causes 50 % of ACE inhibition activity (IC50) was 41 μg/ml. Through LC-QTOF/MS analysis, there were 26 compounds identified in ASP with the following major compounds; 1-galloyl-glucose (21%), feroxin A (12%), nilocitin (10 %), 2,6-di-O-galloyl-β-D-glucose (9%), 1-O-galloylpedun-culagin (7%), 5-desgalloylstachyurin (6 %), and yakuchinone A (6 %). In conclusion, this study showed that ASP which majorly consists of 1-galloyl glucose exhibited significant ACE inhibitory activity. Further bioassay-guided isolation is required to determine whether 1-galloyl glucose is the potential bioactive compound responsible for this activity. Pusat Pengajian Sains Kesihatan 2021-08-16 Conference or Workshop Item PeerReviewed application/pdf en http://irep.iium.edu.my/92974/1/Slide%20NSEPCOH2021%20%2813082021%29.pdf application/pdf en http://irep.iium.edu.my/92974/2/E-BOOK%20NSEPCOH.pdf Tuan Anuar, Tuan Ashraf Faiz and Ismail, Azlini and Omar, Muhammad Nor and Mohamed Suffian, Izzat Fahimuddin and Abdul Hamid, Azzmer Azzar (2021) Angiotensin-converting enzyme inhibition activity and LCMS analysis of Syzygium polyanthum (Wight) Walp leaves. In: National Symposium on Essential Oils, Plants, Cosmetic and Health, School of Health Sciences, Universiti Sains Malaysia. |
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Q Science (General) R Medicine (General) RM Therapeutics. Pharmacology RM300 Drugs and their action Tuan Anuar, Tuan Ashraf Faiz Ismail, Azlini Omar, Muhammad Nor Mohamed Suffian, Izzat Fahimuddin Abdul Hamid, Azzmer Azzar Angiotensin-converting enzyme inhibition activity and LCMS analysis of Syzygium polyanthum (Wight) Walp leaves |
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Syzygium polyanthum is an ethnomedicinal plant that is widely used among the Malay community for treating hypertension. Previous studies have shown that the plant leaves have a significant antihypertensive effect in rats;
however, its mechanism of action and the bioactive compounds is not yet well-studied. One of the possible mechanisms to reduce blood pressure is through inhibiting the angiotensin-converting enzyme (ACE). Therefore, this
study aims to determine the ACE inhibitory activity of S. polyanthum leaves and its phytochemical composition. S. polyanthum leaves were dried, ground, macerated with water in a bath-sonicator, and then freeze-dried to produce
the aqueous S. polyanthum (ASP) leaves extract. The ACE inhibition activity of ASP was tested using Cushman and Cheung method at a range of concentrations from 1 to 1000 µg/ml. The phytochemical composition in ASP was then analysed using Liquid Chromatography Quadrupole Time-of-Flight Mass Spectrometry (LCQTOF/MS). It was found that the concentration of ASP that causes 50 % of ACE inhibition activity (IC50) was 41 μg/ml. Through LC-QTOF/MS analysis, there were 26 compounds identified in ASP with the following major compounds; 1-galloyl-glucose (21%), feroxin A (12%), nilocitin (10 %), 2,6-di-O-galloyl-β-D-glucose (9%), 1-O-galloylpedun-culagin (7%), 5-desgalloylstachyurin (6 %), and yakuchinone A (6 %). In conclusion, this study showed that ASP which majorly consists of 1-galloyl glucose exhibited
significant ACE inhibitory activity. Further bioassay-guided isolation is required to determine whether 1-galloyl glucose is the potential bioactive compound responsible for this activity. |
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Conference or Workshop Item |
author |
Tuan Anuar, Tuan Ashraf Faiz Ismail, Azlini Omar, Muhammad Nor Mohamed Suffian, Izzat Fahimuddin Abdul Hamid, Azzmer Azzar |
author_facet |
Tuan Anuar, Tuan Ashraf Faiz Ismail, Azlini Omar, Muhammad Nor Mohamed Suffian, Izzat Fahimuddin Abdul Hamid, Azzmer Azzar |
author_sort |
Tuan Anuar, Tuan Ashraf Faiz |
title |
Angiotensin-converting enzyme inhibition activity and LCMS analysis of Syzygium polyanthum (Wight) Walp leaves |
title_short |
Angiotensin-converting enzyme inhibition activity and LCMS analysis of Syzygium polyanthum (Wight) Walp leaves |
title_full |
Angiotensin-converting enzyme inhibition activity and LCMS analysis of Syzygium polyanthum (Wight) Walp leaves |
title_fullStr |
Angiotensin-converting enzyme inhibition activity and LCMS analysis of Syzygium polyanthum (Wight) Walp leaves |
title_full_unstemmed |
Angiotensin-converting enzyme inhibition activity and LCMS analysis of Syzygium polyanthum (Wight) Walp leaves |
title_sort |
angiotensin-converting enzyme inhibition activity and lcms analysis of syzygium polyanthum (wight) walp leaves |
publisher |
Pusat Pengajian Sains Kesihatan |
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2021 |
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http://irep.iium.edu.my/92974/1/Slide%20NSEPCOH2021%20%2813082021%29.pdf http://irep.iium.edu.my/92974/2/E-BOOK%20NSEPCOH.pdf http://irep.iium.edu.my/92974/ |
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