Optimization of supercritical fluid extraction of asiaticoside from Centella asiatica using Central Composite Design (CCD)

• Main Authors: Ruslan, Fatin Shazwani, Darnis, Deny Susanti, Bakhtiar, M. Taher, Mohammad, Nurul Farahain
• Format: Article
• Language: English; English
• Published: Taylor & Francis 2020
• Subjects: QD Chemistry
• Online Access: http://irep.iium.edu.my/91754/1/91754_Optimization%20of%20supercritical%20fluid%20extraction.pdf; http://irep.iium.edu.my/91754/2/91754_Optimization%20of%20supercritical%20fluid%20extraction_SCOPUS.pdf; http://irep.iium.edu.my/91754/; https://doi.org/10.1080/01496395.2020.1845208

The extraction of asiaticoside was performed using supercritical fluid extraction to produce highly concentrated extracts. The effects of three factors (co-solvent percentage, pressure, and tempera- ture) on the asiaticoside content were screened using the Two-Level Factorial design and optimized using the Central Composite Design. Asiaticoside was extracted following the treatment combina- tions of three parameters, namely co-solvent (5–15%), temperature (70–90°C), and pressure (1–20 MPa). The optimized extraction parameters were identified at 10%, 10.5 MPa, and 80°C for co- solvent percentage, pressure, and temperature, respectively.