Impedance spectroscopy of adherent mammalian cell culture for biochemical applications: a review
This review paper is focused on various applications of electric cell-substrate impedance sensing (ECIS) using adherent, two-dimensional (2D) mammalian cell culture. The ECIS technique is a non-destructive electrical approach to in vitro monitor in continuous fashion and real time the cell behavi...
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| Main Authors: | , , |
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| Format: | Article |
| Language: | English English English |
| Published: |
Institute of Electrical and Electronics Engineers Inc.
2021
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| Subjects: | |
| Online Access: | http://irep.iium.edu.my/88995/1/88995_Impedance%20Spectroscopy%20of%20Adherent_article.pdf http://irep.iium.edu.my/88995/2/88995_Impedance%20Spectroscopy%20of%20Adherent_scopus.pdf http://irep.iium.edu.my/88995/3/88995_Impedance%20Spectroscopy%20of%20Adherent_wos.pdf http://irep.iium.edu.my/88995/ https://ieeexplore-ieee-org.ezproxy.um.edu.my/stamp/stamp.jsp?tp=&arnumber=9277877 |
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| Summary: | This review paper is focused on various
applications of electric cell-substrate impedance sensing (ECIS) using adherent, two-dimensional (2D) mammalian
cell culture. The ECIS technique is a non-destructive electrical approach to in vitro monitor in continuous fashion and
real time the cell behavior related to attachment, growth,
morphology, motility, proliferation and viability. The use of
living cells as sensorial elements provides the opportunity for
analysis of a large variety of pharmaceutical compounds and
toxicants that affect cellular responses. In this review, we will
cover some of the most important applications of the ECIS
technique such as: impedance of cancer cell, toxicity studies,
and investigation of stem cell using impedance spectroscopy
sensing. Contractile activity of beating cardiomyocytes recorded with ECIS will also be presented. Combination of
impedance sensing and resonant frequency measurements of cell monolayer will be discussed. An innovative stretchable
device with integrated ECIS electrodes will be discussed. Recently, the ECIS technique was combined with machine
learning algorithms to distinguish stem cell proliferation from the differentiation processes. This review demonstrates
that ECIS is a powerful tool, effective to investigate mammalian cell properties and physiological functions and provides
advantages over conventional assays, including simple, rapid and noninvasive cell screening |
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