Inhibition of cystathionine-γ-lyase-mediated hydrogen sulfide production in LPS-stimulated RAW 264.7 macrophages by polyherbal extract
The polyherbal formulation chandraprabha vati (CV) comprising 29 herbs is traditionally used as an anti-inflammatory agent for arthritis and urinary ailments in Indian Siddha medicine. Earlier, we reported that lipopolysaccharide (LPS) induced apoptosis in RAW 264.7 macrophages is mediated by hydr...
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my.iium.irep.881792021-02-01T08:04:13Z http://irep.iium.edu.my/88179/ Inhibition of cystathionine-γ-lyase-mediated hydrogen sulfide production in LPS-stimulated RAW 264.7 macrophages by polyherbal extract Ramasamy, Tamizhselvi Velusamy, Ranjith Kumar Manickam, Venkatraman Kuttulebbai Naina Mohamed Salam, Sirajudeen R Medicine (General) The polyherbal formulation chandraprabha vati (CV) comprising 29 herbs is traditionally used as an anti-inflammatory agent for arthritis and urinary ailments in Indian Siddha medicine. Earlier, we reported that lipopolysaccharide (LPS) induced apoptosis in RAW 264.7 macrophages is mediated by hydrogen sulfide (H2S), a potential target for many inflammatory diseases. Here, we hypothesized that pretreatment with CV decreases H2S level and thereby alleviate the inflammatory conditions induced by LPS in RAW 264.7 macrophages, and this protective effect occurs through alterations in cystathionine-γ-lyase (CSE), a H2S synthesizing enzyme. Accordingly, we evaluated the anti-inflammatory effect of CV in LPS-stimulated RAW 264.7 macrophages in vitro. The CV pretreatment followed by 12 h of LPS-stimulation showed significantly decreased TNF-α, H2S production possibly through CSE gene expression and NF-кB activation compared to the non pretreated macrophages. Our results further confirm NISCAIR Pubications 2021-02 Article PeerReviewed application/pdf en http://irep.iium.edu.my/88179/1/IJEB%2059%282%29%20111-117.pdf Ramasamy, Tamizhselvi and Velusamy, Ranjith Kumar and Manickam, Venkatraman and Kuttulebbai Naina Mohamed Salam, Sirajudeen (2021) Inhibition of cystathionine-γ-lyase-mediated hydrogen sulfide production in LPS-stimulated RAW 264.7 macrophages by polyherbal extract. Indian Journal of Experimental Biology, 59. pp. 111-117. ISSN 0019-5189 E-ISSN 0975-1009 http://nopr.niscair.res.in/bitstream/123456789/56022/1/IJEB%2059%282%29%20111-117.pdf |
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R Medicine (General) Ramasamy, Tamizhselvi Velusamy, Ranjith Kumar Manickam, Venkatraman Kuttulebbai Naina Mohamed Salam, Sirajudeen Inhibition of cystathionine-γ-lyase-mediated hydrogen sulfide production in LPS-stimulated RAW 264.7 macrophages by polyherbal extract |
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The polyherbal formulation chandraprabha vati (CV) comprising 29 herbs is traditionally used as an anti-inflammatory
agent for arthritis and urinary ailments in Indian Siddha medicine. Earlier, we reported that lipopolysaccharide (LPS)
induced apoptosis in RAW 264.7 macrophages is mediated by hydrogen sulfide (H2S), a potential target for many
inflammatory diseases. Here, we hypothesized that pretreatment with CV decreases H2S level and thereby alleviate the
inflammatory conditions induced by LPS in RAW 264.7 macrophages, and this protective effect occurs through alterations
in cystathionine-γ-lyase (CSE), a H2S synthesizing enzyme. Accordingly, we evaluated the anti-inflammatory effect of CV
in LPS-stimulated RAW 264.7 macrophages in vitro. The CV pretreatment followed by 12 h of LPS-stimulation showed
significantly decreased TNF-α, H2S production possibly through CSE gene expression and NF-кB activation compared to
the non pretreated macrophages. Our results further confirm |
format |
Article |
author |
Ramasamy, Tamizhselvi Velusamy, Ranjith Kumar Manickam, Venkatraman Kuttulebbai Naina Mohamed Salam, Sirajudeen |
author_facet |
Ramasamy, Tamizhselvi Velusamy, Ranjith Kumar Manickam, Venkatraman Kuttulebbai Naina Mohamed Salam, Sirajudeen |
author_sort |
Ramasamy, Tamizhselvi |
title |
Inhibition of cystathionine-γ-lyase-mediated hydrogen sulfide production in LPS-stimulated RAW 264.7 macrophages by polyherbal extract |
title_short |
Inhibition of cystathionine-γ-lyase-mediated hydrogen sulfide production in LPS-stimulated RAW 264.7 macrophages by polyherbal extract |
title_full |
Inhibition of cystathionine-γ-lyase-mediated hydrogen sulfide production in LPS-stimulated RAW 264.7 macrophages by polyherbal extract |
title_fullStr |
Inhibition of cystathionine-γ-lyase-mediated hydrogen sulfide production in LPS-stimulated RAW 264.7 macrophages by polyherbal extract |
title_full_unstemmed |
Inhibition of cystathionine-γ-lyase-mediated hydrogen sulfide production in LPS-stimulated RAW 264.7 macrophages by polyherbal extract |
title_sort |
inhibition of cystathionine-γ-lyase-mediated hydrogen sulfide production in lps-stimulated raw 264.7 macrophages by polyherbal extract |
publisher |
NISCAIR Pubications |
publishDate |
2021 |
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http://irep.iium.edu.my/88179/1/IJEB%2059%282%29%20111-117.pdf http://irep.iium.edu.my/88179/ http://nopr.niscair.res.in/bitstream/123456789/56022/1/IJEB%2059%282%29%20111-117.pdf |
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