rK39 Enzyme-linked immunosorbent assay for the diagnosis of Kala-Azar in an endemic zone of Bangladesh
Objective: The diagnostic role of rK39 enzyme linked immunosorbent assay (ELISA) was compared with the buffy-coat Leishmania nested polymerase chain reaction (Ln-PCR) for kala-azar in an endemic zone of Bangladesh. Methodology: This evaluation study included 100 cases comprising of 60 clinically sus...
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2009
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my.iium.irep.872312021-01-07T02:51:29Z http://irep.iium.edu.my/87231/ rK39 Enzyme-linked immunosorbent assay for the diagnosis of Kala-Azar in an endemic zone of Bangladesh Salam, Md. Abdus Mondal, Dinesh Kabir, Mamun Haque, Rashidul QR180 Immunology Objective: The diagnostic role of rK39 enzyme linked immunosorbent assay (ELISA) was compared with the buffy-coat Leishmania nested polymerase chain reaction (Ln-PCR) for kala-azar in an endemic zone of Bangladesh. Methodology: This evaluation study included 100 cases comprising of 60 clinically suspected patients of kala-azar and 40 healthy controls. Anti-rK39 antibody was estimated in plasma by ELISA and buffy coat was utilized to detect Leishmania DNA by Ln-PCR from both patients and controls. The diagnostic role of rK39 ELISA was then compared with Ln-PCR positive cases which were considered as confirmed cases of kala-azar. Results: Out of 60 suspected patients of kala-azar, 58 (96.67%) were found positive in ELISA against 56 (93.33%) of Ln-PCR-positive cases. Further, 55 (98.21%) of ELISA-positive patients were also found positive in Ln-PCR. Voluntary healthy controls (40) included 20 persons from the endemic zone and 20 from non-endemic zone of kala-azar, were all found negative in both Ln-PCR and rK39-ELISA. The sensitivity and specificity of ELISA were found to be 98.21% and 95.65% respectively. Conclusion: rK39-ELISA is a non-invasive serodiagnostic tool for visceral leishmaniasis with very high sensitivity and specificity. Professional Medical Publications 2009 Article PeerReviewed application/pdf en http://irep.iium.edu.my/87231/1/PJMS%20rK39%20ELISA.pdf Salam, Md. Abdus and Mondal, Dinesh and Kabir, Mamun and Haque, Rashidul (2009) rK39 Enzyme-linked immunosorbent assay for the diagnosis of Kala-Azar in an endemic zone of Bangladesh. Pakistan Journal of Mededical Sciences, 25 (4). pp. 635-640. ISSN 1682-024X E-ISSN 1681-715X http://pjms.com.pk/issues/julsep09/pdf/22.article21.pdf |
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QR180 Immunology Salam, Md. Abdus Mondal, Dinesh Kabir, Mamun Haque, Rashidul rK39 Enzyme-linked immunosorbent assay for the diagnosis of Kala-Azar in an endemic zone of Bangladesh |
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Objective: The diagnostic role of rK39 enzyme linked immunosorbent assay (ELISA) was compared with the buffy-coat Leishmania nested polymerase chain reaction (Ln-PCR) for kala-azar in an endemic zone of Bangladesh. Methodology: This evaluation study included 100 cases comprising of 60 clinically suspected patients of kala-azar and 40 healthy controls. Anti-rK39 antibody was estimated in plasma by ELISA and buffy coat was utilized to detect Leishmania DNA by Ln-PCR from both patients and controls. The diagnostic role of rK39 ELISA was then compared with Ln-PCR positive cases which were considered as confirmed cases of kala-azar. Results: Out of 60 suspected patients of kala-azar, 58 (96.67%) were found positive in ELISA against 56 (93.33%) of Ln-PCR-positive cases. Further, 55 (98.21%) of ELISA-positive patients were also found positive in Ln-PCR. Voluntary healthy controls (40) included 20 persons from the
endemic zone and 20 from non-endemic zone of kala-azar, were all found negative in both Ln-PCR and rK39-ELISA. The sensitivity and specificity of ELISA were found to be 98.21% and 95.65% respectively. Conclusion: rK39-ELISA is a non-invasive serodiagnostic tool for visceral leishmaniasis with very high sensitivity and specificity. |
format |
Article |
author |
Salam, Md. Abdus Mondal, Dinesh Kabir, Mamun Haque, Rashidul |
author_facet |
Salam, Md. Abdus Mondal, Dinesh Kabir, Mamun Haque, Rashidul |
author_sort |
Salam, Md. Abdus |
title |
rK39 Enzyme-linked immunosorbent assay for the diagnosis of Kala-Azar in an endemic zone of Bangladesh |
title_short |
rK39 Enzyme-linked immunosorbent assay for the diagnosis of Kala-Azar in an endemic zone of Bangladesh |
title_full |
rK39 Enzyme-linked immunosorbent assay for the diagnosis of Kala-Azar in an endemic zone of Bangladesh |
title_fullStr |
rK39 Enzyme-linked immunosorbent assay for the diagnosis of Kala-Azar in an endemic zone of Bangladesh |
title_full_unstemmed |
rK39 Enzyme-linked immunosorbent assay for the diagnosis of Kala-Azar in an endemic zone of Bangladesh |
title_sort |
rk39 enzyme-linked immunosorbent assay for the diagnosis of kala-azar in an endemic zone of bangladesh |
publisher |
Professional Medical Publications |
publishDate |
2009 |
url |
http://irep.iium.edu.my/87231/1/PJMS%20rK39%20ELISA.pdf http://irep.iium.edu.my/87231/ http://pjms.com.pk/issues/julsep09/pdf/22.article21.pdf |
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