Temporal characteristics of sodium fluorescein in the tear meniscus
Purpose To observe the emission intensity profile of sodium fluorescein in the human tear film as a function of time and concentration. Methods Twenty-two participants with no dry eye signs or symptoms were randomly allocated to receive 1 μL of either a 2 or 10% concentration of fluorescein t...
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Lippincott Williams and Wilkins Ltd.
2017
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my.iium.irep.863692020-12-22T09:09:01Z http://irep.iium.edu.my/86369/ Temporal characteristics of sodium fluorescein in the tear meniscus Markoulli, Maria Md Isa, Nur Amalina Papas, Eric B. Q Science (General) Purpose To observe the emission intensity profile of sodium fluorescein in the human tear film as a function of time and concentration. Methods Twenty-two participants with no dry eye signs or symptoms were randomly allocated to receive 1 μL of either a 2 or 10% concentration of fluorescein to one eye. Images of the inferior tear meniscus were captured at regular intervals over 30 minutes and the process repeated for the other eye with the alternate concentration. Fluorescence intensity was quantified on the basis of the grayscale pixel values in the tear meniscus images. The fluorescein-decay profile over time and between concentrations was determined. Results Peak fluorescence intensity was reached in 3.9 ± 3.0 and 8.7 ± 4.4 minutes after instillation for the 2 and 10% concentrations, respectively. The 10% concentration of fluorescein maintained its peak fluorescence intensity longer than the 2% concentration (about 9 and 2 minutes, respectively). The peak fluorescence intensity was not significantly different between the higher and lower concentrations (44 ± 37 vs. 38 ± 32 units, P = .22). For both concentrations, the observed intensity did not return to baseline levels by the end of the 30-minute observation time. Conclusions The fluorescence intensity of fluorescein in a clinical setting varies with time such that both the onset and duration of maximum brightness are concentration dependent. At low concentration (2%), maximum brightness occurs almost immediately after instillation and lasts about 2 minutes. With a higher concentration (10%), the effective working window is delayed for about 7 to 8 minutes. Irrespective of initial concentration, observable fluorescence remains in the tear film beyond 30 minutes post-instillation. Lippincott Williams and Wilkins Ltd. 2017-02-01 Article PeerReviewed application/pdf en http://irep.iium.edu.my/86369/1/86369_Temporal%20characteristics%20of%20sodium.pdf application/pdf en http://irep.iium.edu.my/86369/2/86369_Temporal%20characteristics%20of%20sodium_SCOPUS.pdf application/pdf en http://irep.iium.edu.my/86369/3/86369_Temporal%20characteristics%20of%20sodium_WOS.pdf Markoulli, Maria and Md Isa, Nur Amalina and Papas, Eric B. (2017) Temporal characteristics of sodium fluorescein in the tear meniscus. Optometry and Vision Science, 94 (2). pp. 166-173. ISSN 1538-9235 https://journals.lww.com/optvissci/subjects/Contact%20Lenses/Abstract/2017/02000/Temporal_Characteristics_of_Sodium_Fluorescein_in.5.aspx 10.1097/OPX.0000000000001012 |
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Q Science (General) Markoulli, Maria Md Isa, Nur Amalina Papas, Eric B. Temporal characteristics of sodium fluorescein in the tear meniscus |
description |
Purpose
To observe the emission intensity profile of sodium fluorescein in the human tear film as a function of time and concentration.
Methods
Twenty-two participants with no dry eye signs or symptoms were randomly allocated to receive 1 μL of either a 2 or 10% concentration of fluorescein to one eye. Images of the inferior tear meniscus were captured at regular intervals over 30 minutes and the process repeated for the other eye with the alternate concentration. Fluorescence intensity was quantified on the basis of the grayscale pixel values in the tear meniscus images. The fluorescein-decay profile over time and between concentrations was determined.
Results
Peak fluorescence intensity was reached in 3.9 ± 3.0 and 8.7 ± 4.4 minutes after instillation for the 2 and 10% concentrations, respectively. The 10% concentration of fluorescein maintained its peak fluorescence intensity longer than the 2% concentration (about 9 and 2 minutes, respectively). The peak fluorescence intensity was not significantly different between the higher and lower concentrations (44 ± 37 vs. 38 ± 32 units, P = .22). For both concentrations, the observed intensity did not return to baseline levels by the end of the 30-minute observation time.
Conclusions
The fluorescence intensity of fluorescein in a clinical setting varies with time such that both the onset and duration of maximum brightness are concentration dependent. At low concentration (2%), maximum brightness occurs almost immediately after instillation and lasts about 2 minutes. With a higher concentration (10%), the effective working window is delayed for about 7 to 8 minutes. Irrespective of initial concentration, observable fluorescence remains in the tear film beyond 30 minutes post-instillation. |
format |
Article |
author |
Markoulli, Maria Md Isa, Nur Amalina Papas, Eric B. |
author_facet |
Markoulli, Maria Md Isa, Nur Amalina Papas, Eric B. |
author_sort |
Markoulli, Maria |
title |
Temporal characteristics of sodium fluorescein in the tear meniscus |
title_short |
Temporal characteristics of sodium fluorescein in the tear meniscus |
title_full |
Temporal characteristics of sodium fluorescein in the tear meniscus |
title_fullStr |
Temporal characteristics of sodium fluorescein in the tear meniscus |
title_full_unstemmed |
Temporal characteristics of sodium fluorescein in the tear meniscus |
title_sort |
temporal characteristics of sodium fluorescein in the tear meniscus |
publisher |
Lippincott Williams and Wilkins Ltd. |
publishDate |
2017 |
url |
http://irep.iium.edu.my/86369/1/86369_Temporal%20characteristics%20of%20sodium.pdf http://irep.iium.edu.my/86369/2/86369_Temporal%20characteristics%20of%20sodium_SCOPUS.pdf http://irep.iium.edu.my/86369/3/86369_Temporal%20characteristics%20of%20sodium_WOS.pdf http://irep.iium.edu.my/86369/ https://journals.lww.com/optvissci/subjects/Contact%20Lenses/Abstract/2017/02000/Temporal_Characteristics_of_Sodium_Fluorescein_in.5.aspx |
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