Neuroprotective effects of co-administration of Coenzyme Q10 and vitamin-E in chronic cerebral hypoperfusion-induced neurodegeneration in rats

• Main Authors: Azimi, Mahmoodullah, Ashour, Abdelkader Elbadawy Abbas, Abd. Fuaat, Azliana, Mohamed, Wael Mohamed Yousef
• Format: Article
• Language: English; English
• Published: Wolters Kluwer Medknow Publications 2020
• Subjects: RM300 Drugs and their action
• Online Access: http://irep.iium.edu.my/83218/1/83218_Neuroprotective%20effects%20of%20co-administration.pdf; http://irep.iium.edu.my/83218/2/83218_Neuroprotective%20effects%20of%20co-administration_SCOPUS.pdf; http://irep.iium.edu.my/83218/; http://www.ijnpnd.com/article.asp?issn=2231-0738;year=2020;volume=10;issue=2;spage=35;epage=42;aulast=Azimi

Alzheimer’s disease (AD) is the most common type of neurodegenerative diseases. Currently, there is no prevention or cure for AD. The potential use of natural antioxidants for prevention and treatment of AD has attracted considerable attention. Here, we used combination of the antioxidants coenzyme Q10 (CoQ10) and vitamin-E (Vit E) for the protection against AD. The current study assessed the neuroprotective effects of combination of CoQ10 with Vit E in Chronic Cerebral Hypoperfusion-induced neurodegeneration (CCH-ND) rat model. After acclimatization, 27 Sprague Dawley rats weighing 220–250 g were divided into six groups; sham control, 2-vessel occlusion (2VO), 2VO+E (treated daily with Vit E, 100 mg/kg, orally following 2VO), CoQ10 (treated daily with CoQ10, 200 mg/kg, orally following 2VO), CoQ10+E (treated with combination of CoQ10 and Vit E, orally following 2VO) and last group was treated with coconut-oil as a vehicle control. On the 8th week, all rats were tested by Morris water maze cognitive test and then euthanized and the hippocampi were isolated. Viable neuronal cell count in the hippocampal region was estimated. The Isoprostane F2 (F2-IsoPs) levels were assessed in the brain homogenates to quantify the oxidative stress status. There was significant difference in neuronal cell death, memory and learning, and F2-Iso level in untreated 2VO group compared to the treated and sham groups. However, there was no statistically significant difference in neuroprotective effects of combination of Vit E with CoQ10 and each one alone. To conclude, combination of the antioxidants (Vit E and CoQ10) improves memory, neuronal cell viability and decreases antioxidant level, same as each antioxidant alone.