Differential expression of miR-101 and miR-744 in nasopharyngeal carcinoma in Pahang state of Malaysia

• Main Authors: Ahmad, Azmir, Ab. Wahid, Haziq, Leman, Wan Ishlah, Othman, Noor Syamila, Abd Rahman, Azlina, Abdullah, Kahairi, Muhammad, Siti Aeshah @ Naznin, Kaderi, Mohd Arifin
• Format: Article
• Language: English; English
• Published: Walailak University 2019
• Subjects: R Medicine (General); RC Internal medicine
• Online Access: http://irep.iium.edu.my/79822/1/79822_Differential%20Expression%20of%20miR-101%20and%20miR-744_article.pdf; http://irep.iium.edu.my/79822/2/79822_Differential%20Expression%20of%20miR-101%20and%20miR-744_scopus.pdf; http://irep.iium.edu.my/79822/; http://wjst.wu.ac.th/index.php/wjst/article/view/6285

Previous study found that microRNA-101 (miR-101) and microRNA-744 (miR-744) were deregulated in head and neck cancers and were implicated in nasopharyngeal carcinoma (NPC) carcinogenesis. Thus, this study aimed to determine the expression of miR-101 and miR-744 in NPC and analyse the utility of these microRNAs (miRNAs) as diagnostic biomarkers. Total RNA was extracted from 31 NPC and 7 non-NPC control formalin-fixed paraffin-embedded (FFPE) samples. Complementary DNA (cDNA) was synthesized from the total RNA and proceeded with quantitative real-time polymerase chain reaction. Differential expression of miR-101 and miR-744 were calculated from quantification cycle (Cq) data using 2-ΔΔCq calculation. The performance of these miRNAs were calculated using receiver operating characteristic (ROC) curve analysis. The differential expression for miR-101 and miR744 were -1.39 (p < 0.05) and 2.48 (p > 0.05), respectively, where the deregulations were consistent with the previous report. The area under curve for miR-101, miR-744 and combination of miR-101 and miR744 were 0.654 (95 % CI: 0.465 - 0.844), 0.588 (95 % CI: 0.368 - 0.808) and 0.626 (95 % CI: 0.481 - 0.771), respectively. However, re-analysis using balanced sample size between NPC and non-NPC control group showed the value decreased to 0.653 (95 % CI: 0.347 - 0.959) for miR-101 but increased to 0.827 (95 % CI: 0.601 - 1.000) for miR-744 and 0.758 (95 % CI: 0.576 - 0.939) for the combination of miR-101 and miR-744, indicating the importance of having a balanced sample size. We have successfully determined the expression of miR-101 and miR-744 in NPC samples. We also demonstrated statistically the utility of these miRNAs as diagnostic biomarkers.