Single-tube, dual channel pentaplexing for the identification of Candida strains associated with human infection.
Invasive candidiasis is one of the most common nosocomial fungal infections worldwide. Delayed implementation of effective antifungal treatment caused by inefficient Candida diagnosis contributes to its notoriously high mortality rates. The availability of better Candida diagnostic tools would posit...
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my.iium.irep.767772020-03-24T06:10:10Z http://irep.iium.edu.my/76777/ Single-tube, dual channel pentaplexing for the identification of Candida strains associated with human infection. Jainlabdin, Mohd Hanif Batra, Ambalika Paredes, Edith Sánchez Hernández Hernández, Francisca Fu, Guoliang Jorge, Tovar-Torres QR Microbiology Invasive candidiasis is one of the most common nosocomial fungal infections worldwide. Delayed implementation of effective antifungal treatment caused by inefficient Candida diagnosis contributes to its notoriously high mortality rates. The availability of better Candida diagnostic tools would positively impact patient outcomes. Here, we report on the development of a single-tube, dual channel pentaplex molecular diagnostic assay based on Multiplex Probe Amplification (MPA) technology. It allows simultaneous identification of C. auris, C. glabrata and C. krusei, at species-level as well as of six additional albicans and non-albicans pathogenic Candida at genus level. The assay overcomes the one-channel one-biomarker limitation of qPCR-based assays. Assay specificities are conferred by unique biomarker probe pairs with characteristic melting temperatures; post-amplification melting curve analysis allows simple identification of the infectious agent. Alerting for the presence of C. auris, the well-characterised multi-drug resistant outbreak strain, will facilitate informed therapy decisions and aid antifungal stewardship. The MPA-Candida assay can also be coupled to a pan-Fungal assay when differentiation between fungal and bacterial infections might be desirable. Its multiplexing capacity, detection range, specificity and sensitivity suggest the potential use of this novel MPA-Candida assay in clinical diagnosis and in the control and management of hospital outbreaks. Nat\ure Publishing Group 2019-10-11 Article PeerReviewed application/pdf en http://irep.iium.edu.my/76777/1/76777_Single-tube%2C%20dual%20channel_WOS.pdf application/pdf en http://irep.iium.edu.my/76777/2/76777_Single-tube%2C%20dual%20channel.pdf Jainlabdin, Mohd Hanif and Batra, Ambalika and Paredes, Edith Sánchez and Hernández Hernández, Francisca and Fu, Guoliang and Jorge, Tovar-Torres (2019) Single-tube, dual channel pentaplexing for the identification of Candida strains associated with human infection. Scientific Reports, 9. pp. 1-14. E-ISSN 2045-2322 https://www.nature.com/articles/s41598-019-51198-6.pdf https://doi.org/10.1038/s41598-019-51198-6 |
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QR Microbiology Jainlabdin, Mohd Hanif Batra, Ambalika Paredes, Edith Sánchez Hernández Hernández, Francisca Fu, Guoliang Jorge, Tovar-Torres Single-tube, dual channel pentaplexing for the identification of Candida strains associated with human infection. |
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Invasive candidiasis is one of the most common nosocomial fungal infections worldwide. Delayed implementation of effective antifungal treatment caused by inefficient Candida diagnosis contributes to its notoriously high mortality rates. The availability of better Candida diagnostic tools would positively impact patient outcomes. Here, we report on the development of a single-tube, dual channel pentaplex molecular diagnostic assay based on Multiplex Probe Amplification (MPA) technology. It allows simultaneous identification of C. auris, C. glabrata and C. krusei, at species-level as well as of six additional albicans and non-albicans pathogenic Candida at genus level. The assay overcomes the one-channel one-biomarker limitation of qPCR-based assays. Assay specificities are conferred by unique biomarker probe pairs with characteristic melting temperatures; post-amplification melting curve analysis allows simple identification of the infectious agent. Alerting for the presence of C. auris, the well-characterised multi-drug resistant outbreak strain, will facilitate informed therapy decisions and aid antifungal stewardship. The MPA-Candida assay can also be coupled to a pan-Fungal assay when differentiation between fungal and bacterial infections might be desirable. Its multiplexing capacity, detection range, specificity and sensitivity suggest the potential use of this novel MPA-Candida assay in clinical diagnosis and in the control and management of hospital outbreaks. |
format |
Article |
author |
Jainlabdin, Mohd Hanif Batra, Ambalika Paredes, Edith Sánchez Hernández Hernández, Francisca Fu, Guoliang Jorge, Tovar-Torres |
author_facet |
Jainlabdin, Mohd Hanif Batra, Ambalika Paredes, Edith Sánchez Hernández Hernández, Francisca Fu, Guoliang Jorge, Tovar-Torres |
author_sort |
Jainlabdin, Mohd Hanif |
title |
Single-tube, dual channel pentaplexing for the identification of Candida strains associated with human infection. |
title_short |
Single-tube, dual channel pentaplexing for the identification of Candida strains associated with human infection. |
title_full |
Single-tube, dual channel pentaplexing for the identification of Candida strains associated with human infection. |
title_fullStr |
Single-tube, dual channel pentaplexing for the identification of Candida strains associated with human infection. |
title_full_unstemmed |
Single-tube, dual channel pentaplexing for the identification of Candida strains associated with human infection. |
title_sort |
single-tube, dual channel pentaplexing for the identification of candida strains associated with human infection. |
publisher |
Nat\ure Publishing Group |
publishDate |
2019 |
url |
http://irep.iium.edu.my/76777/1/76777_Single-tube%2C%20dual%20channel_WOS.pdf http://irep.iium.edu.my/76777/2/76777_Single-tube%2C%20dual%20channel.pdf http://irep.iium.edu.my/76777/ https://www.nature.com/articles/s41598-019-51198-6.pdf https://doi.org/10.1038/s41598-019-51198-6 |
_version_ |
1662753768809168896 |
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13.18916 |