Observation on antioxidant activity from leaves, callus and suspension culture of Justicia gendarusa
This Antioxidant activity and phenolic compound was found in Justicia gendarussa (JG) via total phenolic content (TPC) and α,α-diphenyl-β-pycrilhydrazil hydrate (DPPH) radical scavenging assays. The assays were applied on aqueous and methanolic extracts of leaves, callus culture and cell suspens...
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| Main Authors: | , , , |
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| Format: | Conference or Workshop Item |
| Language: | English |
| Published: |
2011
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| Subjects: | |
| Online Access: | http://irep.iium.edu.my/7466/1/irep.iium.edu.my_7466.pdf http://irep.iium.edu.my/7466/ http://www.iium.edu.my/icbioe/2011/ |
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| Summary: | This Antioxidant activity and phenolic compound was
found in Justicia gendarussa (JG) via total phenolic content
(TPC) and α,α-diphenyl-β-pycrilhydrazil hydrate (DPPH) radical
scavenging assays. The assays were applied on aqueous and
methanolic extracts of leaves, callus culture and cell suspension
culture of JG. Callus was induced from green and matured leaves
of JG cultured on Murashige and Skoog (MS) basal medium
supplemented with different concentrations of 2,4-
dichlorophenoxyacetic acid (2,4-D)or1-Naphthaleneacetic acid
(NAA) and benzylaminopurine (BAP). Callus induced on NAA
(1-Naphthaleneacetic acid) showed higher phenolic content and
antioxidant activity compared to the one induced on 2,4-D.
Highest total phenolic content; 88mg GAE/g was observed from
callus culture induced with NAA and BAP while highest
antioxidant activity (lowest IC50; 8.46x10-3mg/mL) was observed
in the cell suspension |
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