Characterization and in vivo study of decellularized aortic scaffolds using closed sonication system
Extracellular matrix (ECM) based bioscaffolds prepared by decellularization has increasingly emerged in tissue engineering application because it has structural, biochemical, and biomechanical cues that have dramatic effects upon cell behaviors. Therefore, we developed a closed sonication decellu...
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| Main Authors: | , , |
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| Format: | Article |
| Language: | English English |
| Published: |
Taylor & Francis
2019
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| Subjects: | |
| Online Access: | http://irep.iium.edu.my/74164/1/IREP%20AQILAH.pdf http://irep.iium.edu.my/74164/7/74164_Characterization%20and%20in%20vivo%20study%20of%20decellularized%20aortic%20scaffolds%20using%20closed%20sonication%20system_Scopus.pdf http://irep.iium.edu.my/74164/ |
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| Summary: | Extracellular matrix (ECM) based bioscaffolds prepared by decellularization has
increasingly emerged in tissue engineering application because it has structural, biochemical, and
biomechanical cues that have dramatic effects upon cell behaviors. Therefore, we developed a closed
sonication decellularization system to prepare ideal bioscaffolds with minimal adverse effects on the
ECM. The decellularization was achieved at 170 kHz of ultrasound frequency in 0.1% and 2%
Sodium Dodecyl Sulphate (SDS) solution for 10 hours. The immersion treatment as control was
performed to compare the decellularization efficiency with our system. Cell removal and ECM structure were determined by histological staining and biochemical assay. Biomechanical properties
were investigated by the indentation testing to test the stiffness, a residual force and compression of
bioscaffolds. Additionally, in vivo implantation was performed in rat to investigate host tissue
response. Compared to native tissues, histological staining and biochemical assay confirm the
absence of cellularity with preservation of ECM structure. Moreover, sonication treatment has not affected the stiffness [N/mm] and a residual force [N] of the aortic scaffolds except for compression
[%] which 2% SDS significantly decreased compared to native tissues showing higher SDS has
a detrimental effect on ECM structure. Finally, minimal inflammatory response was observed after 1
and 5 weeks of implantation. This study reported that the novelty of our developed closed sonication
system to prepare ideal bioscaffolds for tissue engineering applications. |
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