A flow cell culture apparatus

• Main Authors: Arzmi, Mohd Hafiz, Mohd Nasir, Mohd Hamzah, Idris, Abdul Halim
• Format: Patent
• Language: English; English; English; English
• Published: 2018
• Subjects: QR Microbiology; RK Dentistry
• Online Access: http://irep.iium.edu.my/73637/1/20190108%20%286703%29-Filing%20Reporting.pdf; http://irep.iium.edu.my/73637/2/Specs%20as%20filed%20%286703%29.pdf; http://irep.iium.edu.my/73637/11/Attachment%201%20%28from%20Disclosure%20Form%20V6%29-Mohd%20Hafiz%20Arzmi.pdf; http://irep.iium.edu.my/73637/12/3D%20flow%20cell%20mouth%20model.pdf; http://irep.iium.edu.my/73637/

Bacterial adhesion is an initial and critical step in the oral colonization process. Adhesion processes may include attachment of the bacterial cell to host cells, to components of the extracellular matrix (ECM), to the salivary pellicle on teeth and to soluble factors, as well as to bacterial cells of the same strain (auto-aggregation) or genetically distinct species (co-aggregation). The oral biofilm formation and development, and the inside selection of specific microorganisms have been correlated with oral diseases, such as dental caries, periodontal disease and oral candidiasis. Many of these microorganisms are oral microbiome, that, in particular situations, can express their virulence factors. A better understanding on the mechanisms of these pathologies is necessary in order to elucidate the complex interactions between microorganisms in the biofilm niche and host tissues. Therefore, it is desirable that an apparatus is designed to reproduce the oral environment for in-vitro cell culture study purpose.