Genetic diversity of the orange-spotted grouper (Epinephelus coioides) in Terengganu Malaysia based on mitochondrial cytochrome b sequence data
Orange-spotted groupers (Epinephelus coioides) are marine fish which can be found in the state of Terengganu (in Malaysia) and are economically important. Overfishing and fish farmers’ dependency on wild orange-spotted grouper seed fish have caused a decline in their numbers. Hence, the aim of thi...
Saved in:
Main Authors: | , , |
---|---|
Format: | Article |
Language: | English English |
Published: |
Malaysian Society of Applied Biology
2018
|
Subjects: | |
Online Access: | http://irep.iium.edu.my/69802/1/69802_Genetic%20diversity%20of%20the%20orange-spotted.pdf http://irep.iium.edu.my/69802/7/69802_Genetic%20diversity%20of%20the%20orange-spotted%20grouper_scopus.pdf http://irep.iium.edu.my/69802/ http://www.mabjournal.com/images/47_6_December_2018/47_06_08.pdf |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
id |
my.iium.irep.69802 |
---|---|
record_format |
dspace |
spelling |
my.iium.irep.698022019-02-26T01:34:58Z http://irep.iium.edu.my/69802/ Genetic diversity of the orange-spotted grouper (Epinephelus coioides) in Terengganu Malaysia based on mitochondrial cytochrome b sequence data Waludin, Shaidatul Nadia Mohamed, Ahmad Azfar Mukai, Yukinori QL Zoology SH Aquaculture. Fisheries. Angling Orange-spotted groupers (Epinephelus coioides) are marine fish which can be found in the state of Terengganu (in Malaysia) and are economically important. Overfishing and fish farmers’ dependency on wild orange-spotted grouper seed fish have caused a decline in their numbers. Hence, the aim of this study was to investigate the genetic diversity of wild orange-spotted grouper populations in Terengganu via partial cytochrome b (cyt-b) gene analysis. A total of 60 fish, which were reared from wild seed fish, were collected from farms in Besut and Setiu, Terengganu. Their DNA was extracted using the Qiagen Blood and Tissue kit. PCR amplification was conducted using cyt-b primers, and a sequence of 460 bp in length was obtained for each sample. Next, phylogenetic analysis was performed to study the relationships among the individuals. The haplotype and nucleotide diversities of the populations were investigated to measure genetic diversity. The haplotype diversity (Hd) of E. coioides was relatively low for both Besut (Hd = 0.4161) and Setiu (Hd = 0.8782) populations. The nucleotide diversity (π) for both Besut and Setiu populations was also low. Moreover, 15 haplotypes were identified among the 60 individuals, with 33 individuals sharing a single haplotype (Hap01). These findings similar with a previous study that reported that seed fish dependent can led to a decline in grouper numbers and in turn cause genetic deprivation in the population Malaysian Society of Applied Biology 2018-12-31 Article PeerReviewed application/pdf en http://irep.iium.edu.my/69802/1/69802_Genetic%20diversity%20of%20the%20orange-spotted.pdf application/pdf en http://irep.iium.edu.my/69802/7/69802_Genetic%20diversity%20of%20the%20orange-spotted%20grouper_scopus.pdf Waludin, Shaidatul Nadia and Mohamed, Ahmad Azfar and Mukai, Yukinori (2018) Genetic diversity of the orange-spotted grouper (Epinephelus coioides) in Terengganu Malaysia based on mitochondrial cytochrome b sequence data. Malaysia Applied Biology, 47 (6). pp. 61-68. http://www.mabjournal.com/images/47_6_December_2018/47_06_08.pdf |
institution |
Universiti Islam Antarabangsa Malaysia |
building |
IIUM Library |
collection |
Institutional Repository |
continent |
Asia |
country |
Malaysia |
content_provider |
International Islamic University Malaysia |
content_source |
IIUM Repository (IREP) |
url_provider |
http://irep.iium.edu.my/ |
language |
English English |
topic |
QL Zoology SH Aquaculture. Fisheries. Angling |
spellingShingle |
QL Zoology SH Aquaculture. Fisheries. Angling Waludin, Shaidatul Nadia Mohamed, Ahmad Azfar Mukai, Yukinori Genetic diversity of the orange-spotted grouper (Epinephelus coioides) in Terengganu Malaysia based on mitochondrial cytochrome b sequence data |
description |
Orange-spotted groupers (Epinephelus coioides) are marine fish which can be found in the state of Terengganu (in Malaysia)
and are economically important. Overfishing and fish farmers’ dependency on wild orange-spotted grouper seed fish have
caused a decline in their numbers. Hence, the aim of this study was to investigate the genetic diversity of wild orange-spotted
grouper populations in Terengganu via partial cytochrome b (cyt-b) gene analysis. A total of 60 fish, which were reared from
wild seed fish, were collected from farms in Besut and Setiu, Terengganu. Their DNA was extracted using the Qiagen Blood
and Tissue kit. PCR amplification was conducted using cyt-b primers, and a sequence of 460 bp in length was obtained for
each sample. Next, phylogenetic analysis was performed to study the relationships among the individuals. The haplotype and
nucleotide diversities of the populations were investigated to measure genetic diversity. The haplotype diversity (Hd) of E.
coioides was relatively low for both Besut (Hd = 0.4161) and Setiu (Hd = 0.8782) populations. The nucleotide diversity (π)
for both Besut and Setiu populations was also low. Moreover, 15 haplotypes were identified among the 60 individuals, with
33 individuals sharing a single haplotype (Hap01). These findings similar with a previous study that reported that seed fish
dependent can led to a decline in grouper numbers and in turn cause genetic deprivation in the population |
format |
Article |
author |
Waludin, Shaidatul Nadia Mohamed, Ahmad Azfar Mukai, Yukinori |
author_facet |
Waludin, Shaidatul Nadia Mohamed, Ahmad Azfar Mukai, Yukinori |
author_sort |
Waludin, Shaidatul Nadia |
title |
Genetic diversity of the orange-spotted grouper (Epinephelus coioides) in Terengganu Malaysia based on mitochondrial cytochrome b sequence data |
title_short |
Genetic diversity of the orange-spotted grouper (Epinephelus coioides) in Terengganu Malaysia based on mitochondrial cytochrome b sequence data |
title_full |
Genetic diversity of the orange-spotted grouper (Epinephelus coioides) in Terengganu Malaysia based on mitochondrial cytochrome b sequence data |
title_fullStr |
Genetic diversity of the orange-spotted grouper (Epinephelus coioides) in Terengganu Malaysia based on mitochondrial cytochrome b sequence data |
title_full_unstemmed |
Genetic diversity of the orange-spotted grouper (Epinephelus coioides) in Terengganu Malaysia based on mitochondrial cytochrome b sequence data |
title_sort |
genetic diversity of the orange-spotted grouper (epinephelus coioides) in terengganu malaysia based on mitochondrial cytochrome b sequence data |
publisher |
Malaysian Society of Applied Biology |
publishDate |
2018 |
url |
http://irep.iium.edu.my/69802/1/69802_Genetic%20diversity%20of%20the%20orange-spotted.pdf http://irep.iium.edu.my/69802/7/69802_Genetic%20diversity%20of%20the%20orange-spotted%20grouper_scopus.pdf http://irep.iium.edu.my/69802/ http://www.mabjournal.com/images/47_6_December_2018/47_06_08.pdf |
_version_ |
1643619139218046976 |
score |
13.18916 |