DNA polymorphism of D1S80 locus in modern Malay sample population of Sarawak

• Main Authors: Roslan, Hairul Azman, Azizan, Nur Hafizah, Saat, Rosmawati
• Format: Article
• Language: English
• Published: Faculty of Science and Technology, Universiti Kebangsaan Malaysia 2009
• Subjects: Q Science (General)
• Online Access: http://irep.iium.edu.my/69666/7/69666%20DNA%20Polymorphism%20of%20D1S80%20Locus%20in%20Modern%20Malay.pdf; http://irep.iium.edu.my/69666/; http://www.ukm.my/jsm/english_journals/vol38num2_2009/vol38num2_09page143-147.html

The molecular genetic marker, minisatellite locus D1S80 (1p35-p36), is a highly polymorphic variable number of tandem repeats (VNTR). Its polymorphic nature allows for phylogenetic studies, forensic analysis, genetic maps construction and paternity testing to be performed. A study of the hypervariable locus D1S80 was conducted to determine the allele frequency and distribution of this locus in modern Malay in Sarawak population. The polymerase chain reaction technique was employed and results were analysed on polyacrylamide gel. A total of seventy-six DNA samples of unrelated Malay individuals in UNIMAS were collected and examined. The VNTR analysis of the D1S80 locus demonstrated the presence of 17 alleles in the Malay population. Allele with the size of 577 bp (27 repeats) was determined to be the most common in the sample population with the frequency of 0.1641, followed by allele with the size of 561 bp (26 repeats) and 529 bp (24 repeats) whose frequency is 0.1172 and 0.1094, respectively. The smallest allele is allele with the size of 465 bp (20 repeats) whereas the largest is allele with the size of 753 bp (38 repeats). The sample population exhibited 57.8% heterozygosity. ******************************************************************************************** Penanda genetik molekul, lokus minisatelit D1S80 (1p35-p36), adalah lokus VNTR yang tinggi kepelbagaian rupa bentuknya. Sifat kepelbagaian bentuk ini membolehkan kajian flogenetik, analisis forensik, peta genetik dan kajian penentuan ibu bapa dijalankan. Satu kajian dalam lokus D1S80 telah dijalankan untuk menentukan frekuensi dan penyebaran lokus ini pada populasi Melayu moden di Sarawak. Kajian telah menggunakan teknik amplifkasi rantai polimeras (PCR) dan elektroporesis gel menggunakan media poliakrilamid. Sejumlah 76 sampel DNA dari individu Melayu dalam populasi UNIMAS yang tiada hubungan saudara telah di ambil dan dikaji. Keputusan menunjukkan 17 alel diperoleh dari populasi ini. Alel bersaiz 577 bp (27 ulangan) ialah alel yang biasa dijumpai dengan frekuensi 0.1641 dan diikuti dengan alel bersaiz 561 bp (26 ulangan) dan 529 bp (24 ulangan) dengan frekuensi masing-masing 0.1172 dan 0.1094. Alel terkecil yang dijumpai ialah alel bersaiz 465 bp (20 ulangan) manakala alel yang terbesar ialah alel bersaiz 753 bp (38 ulangan). Populasi ini menunjukkan 56.9% individu heterozigositi.