In vitro cytotoxic and in vivo anxiolytic study of methanolic crude extract of Streculia villosa seeds

• Main Authors: Barua, Soumitra, Banna, Hasanul, Hasan, Md Mahmudul, Azam, Shofiul, Jainul, Mohammed Abdullah, Azad, Abul Kalam, Helal Uddin, A.B.M.
• Format: Article
• Language: English; English
• Published: SILAE (Italo-Latin American Society of Ethnomedicine) 2018
• Subjects: QD Chemistry; RS Pharmacy and materia medica; RS403 Materia Medica-Pharmaceutical Chemistry
• Online Access: http://irep.iium.edu.my/64947/1/64947_In%20vitro%20cytotoxic.pdf; http://irep.iium.edu.my/64947/2/64947_In%20vitro%20cytotoxic_SCOPUS.pdf; http://irep.iium.edu.my/64947/; http://pharmacologyonline.silae.it/files/archives/2018/vol1/PhOL_2018_1_A015_Barua.pdf

We have aimed to evaluate the in vitro cytotoxic and in vivo anxiolytic and sedative activities of the methanolic extract of Sterculia villosa roxb seeds. The dried powder of the seeds was extracted with methanol which was then tested to ascertain the neuropharmacological and cytotoxic potentials. The methanolic extract of Sterculia villosa roxb were subjected to Brine Shrimp lethality bioassay for possible cytotoxicity having LC 50 of 8.672μg/ml. However, fractions produced concentration dependent increase in percent of mortality of Brine Shrimp nauplii indicates the presence of cytotoxic property. We also have studied for possible sedative and anxiolytic activity of the methanolic seed extract of Sterculia villosa roxb in mice. This study includes hole cross, open field, thiopental- sodium induced sleeping time and elevated-plus maze (EPM) tests at the dose of 200 mg/kg while on the peripheral and central nervous system the extract mild to moderately decreased the locomotor activity of mice in hole cross, open field and EPM test. However, the extract moderately has minimized the onset of sleep and slightly has maximized the duration of sleep while administered with thiopental sodium.