Model of critical size defect in the New Zealand White rabbit's tibia

• Main Authors: Che Seman, Che Nor Zarida, Zakaria@Mohamad, Zamzuri, Sharifudin, Mohd Ariff, Che Ahmad, Aminudin, Awang, Mohd Shukrimi, Mohd Yusof, Nazri, Buyong, Zunariah
• Format: Article
• Language: English; English; English
• Published: 2018
• Subjects: R Medicine (General)
• Online Access: http://irep.iium.edu.my/63258/1/Vol17-No1-p013018%20-%20Model%20of%20critical%20size%20defect.pdf; http://irep.iium.edu.my/63258/7/63258_Model%20of%20critical%20size%20defect%20in%20the%20New%20Zealand%20White_scopus.pdf; http://irep.iium.edu.my/63258/13/63258_Model%20of%20critical%20size%20defect%20in%20the%20New%20Zealand%20White_WoS.pdf; http://irep.iium.edu.my/63258/; http://iiumedic.net/imjm/v1/

Introduction: Critical size defects (CSD) in the long bones of New Zealand White rabbits (Oryctolagus cuniculus) have been used for years as an experimental model for investigation of the effectiveness of a new bone substitute material. There are varieties of protocols available in the literature. This technical note attempts to present an alternative surgical technique of a CSD in the New Zealand white rabbit tibia. Methods: Thirty-nine New Zealand White rabbits were used in this study. A CSD of approximately 4.5 mm (width) X 9.0 mm (length) was surgically drilled at the proximal tibial metaphysis, approximately 1 cm from the knee joint. The surrounding of soft tissue was repositioned and sutured layer by layer with bioabsorbable surgical suture. Two x-rays of anteroposterior and lateral were taken before assessed under computed tomography scan at 6, 12 and 24 weeks. Results: This alternative method created CSD with less bleeding from the muscle observed. No mortality or other surgical complications observed within 6 weeks, 12 weeks and 24 weeks following surgery. Conclusion: A simple and safe method for performing CSD was demonstrated and recommended as an alternative approach for surgery on New Zealand White rabbits.