Optimization of ultraviolet ozone treatment process for improvement of polycaprolactone (PCL) microcarrier performance
Growing cells on microcarriers may have overcome the limitation of conventional cell culture system. However, the surface functionality of certain polymeric microcarriers for effective cell attachment and growth remains a challenge. Polycaprolactone (PCL), a biodegradable polymer has received consid...
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my.iium.irep.631842018-03-28T06:58:29Z http://irep.iium.edu.my/63184/ Optimization of ultraviolet ozone treatment process for improvement of polycaprolactone (PCL) microcarrier performance Samsudin, Nurhusna Hashim, Yumi Zuhanis Has-Yun Arifin, Mohd Azmir Mel, Maizirwan Mohd. Salleh, Hamzah Sopyan, Iis Jimat, Dzun Noraini TA164 Bioengineering TA401 Materials of engineering and construction Growing cells on microcarriers may have overcome the limitation of conventional cell culture system. However, the surface functionality of certain polymeric microcarriers for effective cell attachment and growth remains a challenge. Polycaprolactone (PCL), a biodegradable polymer has received considerable attention due to its good mechanical properties and degradation rate. The drawback is the non-polar hydrocarbon moiety which makes it not readily suitable for cell attachment. This report concerns the modification of PCL microcarrier surface (introduction of functional oxygen groups) using ultraviolet irradiation and ozone (UV/O3) system and investigation of the effects of ozone concentration, the amount of PCL and exposure time; where the optimum conditions were found to be at 60,110.52 ppm, 5.5 g PCL and 60 min, respectively. The optimum concentration of carboxyl group (COOH) absorbed on the surface was 1495.92 nmol/g and the amount of gelatin immobilized was 320 ± 0.9 µg/g on UV/O3 treated microcarriers as compared to the untreated (26.83 ± 3 µg/g) microcarriers. The absorption of functional oxygen groups on the surface and the immobilized gelatin was confirmed with the attenuated total reflectance Fourier transformed infrared spectroscopy (ATR-FTIR) and the enhancement of hydrophilicity of the surface was confirmed using water contact angle measurement which decreased (86.93°–49.34°) after UV/O3 treatment and subsequently after immobilization of gelatin. The attachment and growth kinetics for HaCaT skin keratinocyte cells showed that adhesion occurred much more rapidly for oxidized surfaces and gelatin immobilized surface as compared to untreated PCL. © 2017, Springer Science+Business Media Dordrecht. Springer Netherlands 2017 Article REM application/pdf en http://irep.iium.edu.my/63184/1/63184_Optmization%20of%20ultraviolet%20ozone%20treatment.pdf application/pdf en http://irep.iium.edu.my/63184/2/63184_Optmization%20of%20ultraviolet%20ozone%20treatment_SCOPUS.pdf Samsudin, Nurhusna and Hashim, Yumi Zuhanis Has-Yun and Arifin, Mohd Azmir and Mel, Maizirwan and Mohd. Salleh, Hamzah and Sopyan, Iis and Jimat, Dzun Noraini (2017) Optimization of ultraviolet ozone treatment process for improvement of polycaprolactone (PCL) microcarrier performance. Cytotechnology, 69. pp. 601-616. ISSN 0920-9069 https://link.springer.com/article/10.1007%2Fs10616-017-0071-x |
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TA164 Bioengineering TA401 Materials of engineering and construction Samsudin, Nurhusna Hashim, Yumi Zuhanis Has-Yun Arifin, Mohd Azmir Mel, Maizirwan Mohd. Salleh, Hamzah Sopyan, Iis Jimat, Dzun Noraini Optimization of ultraviolet ozone treatment process for improvement of polycaprolactone (PCL) microcarrier performance |
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Growing cells on microcarriers may have overcome the limitation of conventional cell culture system. However, the surface functionality of certain polymeric microcarriers for effective cell attachment and growth remains a challenge. Polycaprolactone (PCL), a biodegradable polymer has received considerable attention due to its good mechanical properties and degradation rate. The drawback is the non-polar hydrocarbon moiety which makes it not readily suitable for cell attachment. This report concerns the modification of PCL microcarrier surface (introduction of functional oxygen groups) using ultraviolet irradiation and ozone (UV/O3) system and investigation of the effects of ozone concentration, the amount of PCL and exposure time; where the optimum conditions were found to be at 60,110.52 ppm, 5.5 g PCL and 60 min, respectively. The optimum concentration of carboxyl group (COOH) absorbed on the surface was 1495.92 nmol/g and the amount of gelatin immobilized was 320 ± 0.9 µg/g on UV/O3 treated microcarriers as compared to the untreated (26.83 ± 3 µg/g) microcarriers. The absorption of functional oxygen groups on the surface and the immobilized gelatin was confirmed with the attenuated total reflectance Fourier transformed infrared spectroscopy (ATR-FTIR) and the enhancement of hydrophilicity of the surface was confirmed using water contact angle measurement which decreased (86.93°–49.34°) after UV/O3 treatment and subsequently after immobilization of gelatin. The attachment and growth kinetics for HaCaT skin keratinocyte cells showed that adhesion occurred much more rapidly for oxidized surfaces and gelatin immobilized surface as compared to untreated PCL. © 2017, Springer Science+Business Media Dordrecht. |
format |
Article |
author |
Samsudin, Nurhusna Hashim, Yumi Zuhanis Has-Yun Arifin, Mohd Azmir Mel, Maizirwan Mohd. Salleh, Hamzah Sopyan, Iis Jimat, Dzun Noraini |
author_facet |
Samsudin, Nurhusna Hashim, Yumi Zuhanis Has-Yun Arifin, Mohd Azmir Mel, Maizirwan Mohd. Salleh, Hamzah Sopyan, Iis Jimat, Dzun Noraini |
author_sort |
Samsudin, Nurhusna |
title |
Optimization of ultraviolet ozone treatment process for improvement of polycaprolactone (PCL) microcarrier performance |
title_short |
Optimization of ultraviolet ozone treatment process for improvement of polycaprolactone (PCL) microcarrier performance |
title_full |
Optimization of ultraviolet ozone treatment process for improvement of polycaprolactone (PCL) microcarrier performance |
title_fullStr |
Optimization of ultraviolet ozone treatment process for improvement of polycaprolactone (PCL) microcarrier performance |
title_full_unstemmed |
Optimization of ultraviolet ozone treatment process for improvement of polycaprolactone (PCL) microcarrier performance |
title_sort |
optimization of ultraviolet ozone treatment process for improvement of polycaprolactone (pcl) microcarrier performance |
publisher |
Springer Netherlands |
publishDate |
2017 |
url |
http://irep.iium.edu.my/63184/1/63184_Optmization%20of%20ultraviolet%20ozone%20treatment.pdf http://irep.iium.edu.my/63184/2/63184_Optmization%20of%20ultraviolet%20ozone%20treatment_SCOPUS.pdf http://irep.iium.edu.my/63184/ https://link.springer.com/article/10.1007%2Fs10616-017-0071-x |
_version_ |
1643616316814262272 |
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13.19449 |