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Study of immobilized bovine and fish gelatin on carboxyl containing Polystyrene beads for Vero cell culture

The aim of this study is to develop a polymeric microcarrier (MC) for obtaining a higher cell density of Vero cells in spinner vessel. Different types of gelatin; bovine (BG), fish (FG), cationized bovine (CBG) and cationized fish (CFG) were covalently immobilized on carboxyl group containing po...

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Main Authors: Ahmad Nor, Yusilawati, Mel, Maizirwan, Mohd. Salleh, Hamzah, Ng, Kim Hooi, Wong, C. S.
Format: Article
Language:English
Published: Malaysian Medical Associations 2010
Subjects:
TP248.13 Biotechnology
Online Access:http://irep.iium.edu.my/5950/1/Paper_MJM_2010-Yusilawati.pdf
http://irep.iium.edu.my/5950/
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spelling my.iium.irep.59502021-07-06T01:53:35Z http://irep.iium.edu.my/5950/ Study of immobilized bovine and fish gelatin on carboxyl containing Polystyrene beads for Vero cell culture Ahmad Nor, Yusilawati Mel, Maizirwan Mohd. Salleh, Hamzah Ng, Kim Hooi Wong, C. S. TP248.13 Biotechnology The aim of this study is to develop a polymeric microcarrier (MC) for obtaining a higher cell density of Vero cells in spinner vessel. Different types of gelatin; bovine (BG), fish (FG), cationized bovine (CBG) and cationized fish (CFG) were covalently immobilized on carboxyl group containing polystyrene (COOH-PS). Their performance was evaluated by the cell loading test in Vero cell culture as carrier system. It was found that Vero cell has better performance on BG with highest maximum cell number at 1.45x105 cell/ml with good microscopic and suspension ability; easy sampling and easy cell recovery. Malaysian Medical Associations 2010 Article PeerReviewed application/pdf en http://irep.iium.edu.my/5950/1/Paper_MJM_2010-Yusilawati.pdf Ahmad Nor, Yusilawati and Mel, Maizirwan and Mohd. Salleh, Hamzah and Ng, Kim Hooi and Wong, C. S. (2010) Study of immobilized bovine and fish gelatin on carboxyl containing Polystyrene beads for Vero cell culture. Medical Journal of Malaysia, 65. pp. 26-27. ISSN 0300-5283
institution Universiti Islam Antarabangsa Malaysia
building IIUM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider International Islamic University Malaysia
content_source IIUM Repository (IREP)
url_provider http://irep.iium.edu.my/
language English
topic TP248.13 Biotechnology
spellingShingle TP248.13 Biotechnology
Ahmad Nor, Yusilawati
Mel, Maizirwan
Mohd. Salleh, Hamzah
Ng, Kim Hooi
Wong, C. S.
Study of immobilized bovine and fish gelatin on carboxyl containing Polystyrene beads for Vero cell culture
description The aim of this study is to develop a polymeric microcarrier (MC) for obtaining a higher cell density of Vero cells in spinner vessel. Different types of gelatin; bovine (BG), fish (FG), cationized bovine (CBG) and cationized fish (CFG) were covalently immobilized on carboxyl group containing polystyrene (COOH-PS). Their performance was evaluated by the cell loading test in Vero cell culture as carrier system. It was found that Vero cell has better performance on BG with highest maximum cell number at 1.45x105 cell/ml with good microscopic and suspension ability; easy sampling and easy cell recovery.
format Article
author Ahmad Nor, Yusilawati
Mel, Maizirwan
Mohd. Salleh, Hamzah
Ng, Kim Hooi
Wong, C. S.
author_facet Ahmad Nor, Yusilawati
Mel, Maizirwan
Mohd. Salleh, Hamzah
Ng, Kim Hooi
Wong, C. S.
author_sort Ahmad Nor, Yusilawati
title Study of immobilized bovine and fish gelatin on carboxyl containing Polystyrene beads for Vero cell culture
title_short Study of immobilized bovine and fish gelatin on carboxyl containing Polystyrene beads for Vero cell culture
title_full Study of immobilized bovine and fish gelatin on carboxyl containing Polystyrene beads for Vero cell culture
title_fullStr Study of immobilized bovine and fish gelatin on carboxyl containing Polystyrene beads for Vero cell culture
title_full_unstemmed Study of immobilized bovine and fish gelatin on carboxyl containing Polystyrene beads for Vero cell culture
title_sort study of immobilized bovine and fish gelatin on carboxyl containing polystyrene beads for vero cell culture
publisher Malaysian Medical Associations
publishDate 2010
url http://irep.iium.edu.my/5950/1/Paper_MJM_2010-Yusilawati.pdf
http://irep.iium.edu.my/5950/
_version_ 1705056445126410240
score 13.154949

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