Optimization of operating conditions of rotary drum bioreactor for lignin peroxidise production by solid state

• Main Authors: Alam, Md. Zahangir, Muyibi, Suleyman Aremu, Al-Mamun, Abdullah, Qudsieh, Isam Y., Suardi, M.
• Format: Conference or Workshop Item
• Language: English
• Published: 2007
• Subjects: TP Chemical technology; TP248.13 Biotechnology
• Online Access: http://irep.iium.edu.my/5855/1/31FCh_42.pdf; http://irep.iium.edu.my/5855/

Solid state bioconversion of empty fruit bunches (EFB) for lignin peroxidase (LiP) by Phanerocheate chrysosporium through optimum operating conditions of a rotary drum bioreactor (RDB) was investigated. The study was carried out in the 50L of RDB fabricated locally for 6 days of treatment. The process conditions such as initial pH of 5.5, inoculum of 5% and moisture content of 60% obtained from previous study were applied for this study. The operating conditions i.e. aeration and agitation rate for the rotary drum bioreactor were optimized by using fractional factorial design. A regression model was developed using experimental data with the consideration of linear, quadratic and interactions effects. The maximum production of LiP was 475.8 U/L while the theoretical yield was 439.6 with the optimum aeration and agitation of 30 l/min and 15 rpm respectively using RDB by solid state bioconversion.