Statistical screening of medium components affecting lipase production using palm oil mill effluent by Penicillium citrinum

• Main Authors: Salihu, Aliyu, Alam, Md. Zahangir, Abdul Karim, Mohamed Ismail, Hamzah, Mohd. Salleh
• Format: Conference or Workshop Item
• Language: English; English
• Published: 2011
• Subjects: TP Chemical technology; TP248.13 Biotechnology
• Online Access: http://irep.iium.edu.my/5687/1/ICBioE_2011_Salihu_et_al.pdf; http://irep.iium.edu.my/5687/4/statistical_screening_of_medium_components_affecting_lipase_production_using_palm_oil_mill_effluent_by_penicillium_citrinium.pdf; http://irep.iium.edu.my/5687/; http://www.iium.edu.my/icbioe/2011/

A number of medium components influencing lipase production by Penicillium citrinum (ATCC 42799) were studied using palm oil mill effluent as the basal medium. The medium components (peptone, yeast extract, malt extract, NH4Cl, NaNO3, KH2PO4, CaCl2, MgSO4, olive oil and tween-80) were analyzed in twelve experimental trials using Plackett–Burman design. The most significant components affecting lipase production were found to be tween-80, peptone, yeast extract, malt extract and NaNO3 at p < 0.05. The results indicated the efficiency of using PB design for screening processes. However, optimal concentration of the significant components can be determined by further statistical analysis.