Identification of Beta Human Chorionic Gonadotrophin (BHcg) expression in frozen cleavage embryo of in vitro fertilization (Ivf) patients: an essential approach for better outcome towards human being inheritance in line with Maqasid Syari’ah concept

• Main Authors: Mat Yusof, Afzan, Md Isa, Muhammad Lokman, Ramli, Roszaman, Abdul Wahab, Azantee Yazmie
• Format: Conference or Workshop Item
• Language: English; English
• Published: International Islamic University Malaysia 2016
• Subjects: Q Science (General)
• Online Access: http://irep.iium.edu.my/56824/1/Conference%202WCII2016%201.pdf; http://irep.iium.edu.my/56824/12/56824_Identification%20of%20beta%20human%20chorionic%20gonadotrophin_SCOPUS%202016.pdf; http://irep.iium.edu.my/56824/; http://www.iium.edu.my/wcii/

Expression of beta-human chorionic gonadotropin (BHCG) mRNA in early cleavage cell stages is important in predicting embryo viability at blastocyst stage of development. Embryo viability is crucial for in vitro fertilization (IVF) treatment to be succeeded in terms of increment of implantation and pregnancy rate. In order to establish fertilization outside the human body, we should ensure the condition is optimum and as closer as inside the body in term of time and also the chemical processes that involved including hormones and growth factors. Imam Muslim narrated from Hudhayfa ibn Asad that the Prophet Muhammad (s.a.w.) said: “After the sperm-and-ovum drop (nut.fa) has been [in the uterus] fortytwo days, Allah sends it an angel that gives it form and fashions its hearing, sight, skin, flesh, and skeleton.” Therefore the aim of this study is to find the significant association of BHCG expression with early cell stage cleavages and its relationship as predictive marker for potential embryo implantation. Our study focuses on leftover frozen embryos from eight patients consists of six pregnant patients and two non-pregnant patients. We assessed the human BHCG mRNA expression at different cell cleavage stages in these frozen embryos using reverse transcriptase-polymerase chain reaction (RT-PCR). Our results have shown three out of eight patients with five to ten cells of blastomeres were expressed with BHCG. This study indicated that BHCG was expressed on frozethawed late cleavage stage of embryos in IVF patients.