Purification and characterization of extracellular lipase from Candida cylindracea using palm oil mill effluent supplemented medium

• Main Authors: Alam, Md. Zahangir, Salihu, Aliyu, Abdul Karim, Mohamed Ismail, Hamzah, Mohd. Salleh
• Format: Conference or Workshop Item
• Language: English
• Published: 2011
• Subjects: TP Chemical technology; TP248.13 Biotechnology
• Online Access: http://irep.iium.edu.my/5629/1/article_SOMChe-ICCEIB_2011.pdf; http://irep.iium.edu.my/5629/; http://icceib.ump.edu.my/

Production of Candida cylindracea lipase was optimized and highest lipase production was achieved after 36 hr of fermentation using palm oil mill effluent supplemented medium. The extracellular lipase was purified by DEAE-sepharose and Sephacryl S-200, exhibiting a single band with molecular mass of...