Enzyme immunoassay for the detection of porcine gelatine in edible bird’s nests

• Main Authors: Tukiran, Nur Azira, Ismail, Amin, Mustafa, Shuhaimi, Hamid, Muhajir
• Format: Article
• Language: English; English
• Published: Taylor & Francis Group 2015
• Subjects: TP Chemical technology
• Online Access: http://irep.iium.edu.my/55414/7/55414-edited.pdf; http://irep.iium.edu.my/55414/8/55414-Enzyme%20immunoassay%20for%20the%20detection%20of%20porcine%20gelatine%20in%20edible%20bird%E2%80%99s%20nests_SCOPUS.pdf; http://irep.iium.edu.my/55414/; http://www.tandfonline.com/doi/abs/10.1080/19440049.2015.1039605

Porcine gelatine is a common adulterant found in edible bird’s nests (EBNs) used to increase the net weight prior to sale. This study aimed to develop indirect enzyme-linked immunosorbent assays (ELISAs) for porcine gelatine adulteration using anti-peptide polyclonal antibodies. Three indirect ELISAs were developed (PAB1, 2 and 3), which had limits of detection (LODs) of 0.12, 0.10 and 0.11 μg g–1, respectively. When applied to standard solutions of porcine gelatine, the inter- and intra-assays showed coefficients of variation (CVs) less than 20% and were able to detect at least 0.5 ng μg–1 (0.05%) porcine gelatine in spiked samples. The proposed ELISA offers attractions for quality control in the EBN industry.