Enzyme immunoassay for the detection of porcine gelatine in edible bird’s nests

Porcine gelatine is a common adulterant found in edible bird’s nests (EBNs) used to increase the net weight prior to sale. This study aimed to develop indirect enzyme-linked immunosorbent assays (ELISAs) for porcine gelatine adulteration using anti-peptide polyclonal antibodies. Three indirect ELI...

وصف كامل

محفوظ في:
التفاصيل البيبلوغرافية
المؤلفون الرئيسيون: Tukiran, Nur Azira, Ismail, Amin, Mustafa, Shuhaimi, Hamid, Muhajir
التنسيق: مقال
اللغة:English
English
منشور في: Taylor & Francis Group 2015
الموضوعات:
الوصول للمادة أونلاين:http://irep.iium.edu.my/55414/7/55414-edited.pdf
http://irep.iium.edu.my/55414/8/55414-Enzyme%20immunoassay%20for%20the%20detection%20of%20porcine%20gelatine%20in%20edible%20bird%E2%80%99s%20nests_SCOPUS.pdf
http://irep.iium.edu.my/55414/
http://www.tandfonline.com/doi/abs/10.1080/19440049.2015.1039605
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spelling my.iium.irep.554142017-07-18T00:40:02Z http://irep.iium.edu.my/55414/ Enzyme immunoassay for the detection of porcine gelatine in edible bird’s nests Tukiran, Nur Azira Ismail, Amin Mustafa, Shuhaimi Hamid, Muhajir TP Chemical technology Porcine gelatine is a common adulterant found in edible bird’s nests (EBNs) used to increase the net weight prior to sale. This study aimed to develop indirect enzyme-linked immunosorbent assays (ELISAs) for porcine gelatine adulteration using anti-peptide polyclonal antibodies. Three indirect ELISAs were developed (PAB1, 2 and 3), which had limits of detection (LODs) of 0.12, 0.10 and 0.11 μg g–1, respectively. When applied to standard solutions of porcine gelatine, the inter- and intra-assays showed coefficients of variation (CVs) less than 20% and were able to detect at least 0.5 ng μg–1 (0.05%) porcine gelatine in spiked samples. The proposed ELISA offers attractions for quality control in the EBN industry. Taylor & Francis Group 2015 Article REM application/pdf en http://irep.iium.edu.my/55414/7/55414-edited.pdf application/pdf en http://irep.iium.edu.my/55414/8/55414-Enzyme%20immunoassay%20for%20the%20detection%20of%20porcine%20gelatine%20in%20edible%20bird%E2%80%99s%20nests_SCOPUS.pdf Tukiran, Nur Azira and Ismail, Amin and Mustafa, Shuhaimi and Hamid, Muhajir (2015) Enzyme immunoassay for the detection of porcine gelatine in edible bird’s nests. Food Additives and Contaminants: Part A, 32 (7). pp. 1023-1028. ISSN 1944-0049 http://www.tandfonline.com/doi/abs/10.1080/19440049.2015.1039605 10.1080/19440049.2015.1039605
institution Universiti Islam Antarabangsa Malaysia
building IIUM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider International Islamic University Malaysia
content_source IIUM Repository (IREP)
url_provider http://irep.iium.edu.my/
language English
English
topic TP Chemical technology
spellingShingle TP Chemical technology
Tukiran, Nur Azira
Ismail, Amin
Mustafa, Shuhaimi
Hamid, Muhajir
Enzyme immunoassay for the detection of porcine gelatine in edible bird’s nests
description Porcine gelatine is a common adulterant found in edible bird’s nests (EBNs) used to increase the net weight prior to sale. This study aimed to develop indirect enzyme-linked immunosorbent assays (ELISAs) for porcine gelatine adulteration using anti-peptide polyclonal antibodies. Three indirect ELISAs were developed (PAB1, 2 and 3), which had limits of detection (LODs) of 0.12, 0.10 and 0.11 μg g–1, respectively. When applied to standard solutions of porcine gelatine, the inter- and intra-assays showed coefficients of variation (CVs) less than 20% and were able to detect at least 0.5 ng μg–1 (0.05%) porcine gelatine in spiked samples. The proposed ELISA offers attractions for quality control in the EBN industry.
format Article
author Tukiran, Nur Azira
Ismail, Amin
Mustafa, Shuhaimi
Hamid, Muhajir
author_facet Tukiran, Nur Azira
Ismail, Amin
Mustafa, Shuhaimi
Hamid, Muhajir
author_sort Tukiran, Nur Azira
title Enzyme immunoassay for the detection of porcine gelatine in edible bird’s nests
title_short Enzyme immunoassay for the detection of porcine gelatine in edible bird’s nests
title_full Enzyme immunoassay for the detection of porcine gelatine in edible bird’s nests
title_fullStr Enzyme immunoassay for the detection of porcine gelatine in edible bird’s nests
title_full_unstemmed Enzyme immunoassay for the detection of porcine gelatine in edible bird’s nests
title_sort enzyme immunoassay for the detection of porcine gelatine in edible bird’s nests
publisher Taylor & Francis Group
publishDate 2015
url http://irep.iium.edu.my/55414/7/55414-edited.pdf
http://irep.iium.edu.my/55414/8/55414-Enzyme%20immunoassay%20for%20the%20detection%20of%20porcine%20gelatine%20in%20edible%20bird%E2%80%99s%20nests_SCOPUS.pdf
http://irep.iium.edu.my/55414/
http://www.tandfonline.com/doi/abs/10.1080/19440049.2015.1039605
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