Monoclonal antibody production: media optimization for enhancement the cell viability of hybridoma cell
Media optimization of RCI hybridoma cell culture for monoclonal antibody production was carried out in T -Flask experiment. The three identified important variables to affect the cell viability were studied. By using Central Composite Design of Response Surface Methodology(STATISTICA v 6.1) has show...
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my.iium.irep.54762017-08-02T08:59:49Z http://irep.iium.edu.my/5476/ Monoclonal antibody production: media optimization for enhancement the cell viability of hybridoma cell Mel, Maizirwan Mat Saad, Maizura Hashim, Yumi Zuhanis Has-Yun Mohamed Salleh, Moharnad Ramlan TP248.13 Biotechnology Media optimization of RCI hybridoma cell culture for monoclonal antibody production was carried out in T -Flask experiment. The three identified important variables to affect the cell viability were studied. By using Central Composite Design of Response Surface Methodology(STATISTICA v 6.1) has shown that cell viability was mainly affected by glutamine, serum and NaC03 concentration, respectively. Among the 16 nillS tested, Rllll 11 indicated the best viability of the cell (>80% for five days). The critical values were obtained at 13.5, 1.68 and 0.87% for serum, glutamine and NaC03, respectively. These data were very significant where the p-values obtained for glutamine, serum and NaCO, were 0.000069, 0.003968 and 0.342151 (R' ~ 0.95476 and R Adj. ~ 0.88691), respectively. Asian Network for Scientific Information 2008 Article REM application/pdf en http://irep.iium.edu.my/5476/1/1957-AJSR_%28Published_On-Line%29.pdf Mel, Maizirwan and Mat Saad, Maizura and Hashim, Yumi Zuhanis Has-Yun and Mohamed Salleh, Moharnad Ramlan (2008) Monoclonal antibody production: media optimization for enhancement the cell viability of hybridoma cell. Asian Journal of Scientific Research, 1 (5). pp. 525-531. ISSN 1992-1454 http://docsdrive.com/pdfs/ansinet/ajsr/2008/525-531.pdf |
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TP248.13 Biotechnology Mel, Maizirwan Mat Saad, Maizura Hashim, Yumi Zuhanis Has-Yun Mohamed Salleh, Moharnad Ramlan Monoclonal antibody production: media optimization for enhancement the cell viability of hybridoma cell |
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Media optimization of RCI hybridoma cell culture for monoclonal antibody production was carried out in T -Flask experiment. The three identified important variables to affect the cell viability were studied. By using Central Composite Design of Response Surface Methodology(STATISTICA v 6.1) has shown that cell viability was mainly affected by glutamine, serum and NaC03 concentration, respectively. Among the 16 nillS tested, Rllll 11 indicated the best viability of the cell (>80% for five days). The critical values were obtained at 13.5, 1.68 and 0.87% for serum, glutamine and NaC03, respectively. These data were very significant where the p-values obtained for glutamine, serum and NaCO, were 0.000069, 0.003968 and 0.342151 (R' ~ 0.95476 and R Adj. ~ 0.88691), respectively. |
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Article |
author |
Mel, Maizirwan Mat Saad, Maizura Hashim, Yumi Zuhanis Has-Yun Mohamed Salleh, Moharnad Ramlan |
author_facet |
Mel, Maizirwan Mat Saad, Maizura Hashim, Yumi Zuhanis Has-Yun Mohamed Salleh, Moharnad Ramlan |
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Mel, Maizirwan |
title |
Monoclonal antibody production: media optimization for
enhancement the cell viability of hybridoma cell |
title_short |
Monoclonal antibody production: media optimization for
enhancement the cell viability of hybridoma cell |
title_full |
Monoclonal antibody production: media optimization for
enhancement the cell viability of hybridoma cell |
title_fullStr |
Monoclonal antibody production: media optimization for
enhancement the cell viability of hybridoma cell |
title_full_unstemmed |
Monoclonal antibody production: media optimization for
enhancement the cell viability of hybridoma cell |
title_sort |
monoclonal antibody production: media optimization for
enhancement the cell viability of hybridoma cell |
publisher |
Asian Network for Scientific Information |
publishDate |
2008 |
url |
http://irep.iium.edu.my/5476/1/1957-AJSR_%28Published_On-Line%29.pdf http://irep.iium.edu.my/5476/ http://docsdrive.com/pdfs/ansinet/ajsr/2008/525-531.pdf |
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1643605549211713536 |
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