Coeliac disease: immunogenicity studies of barley hordein and rye secalin-derived peptides

Coeliac disease (CD) is an inflammatory disorder of the small intestine. It includes aberrant adaptive immunity with presentation of CD toxic gluten peptides by HLA-DQ2 or DQ8 molecules to gluten-sensitive T cells. A ω-gliadin/C-hordein peptide (QPFPQPEQPFPW) and a rye-derived secalin peptide (QPFPQ...

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Main Authors: Abdul Wahab, Widya, Šuligoj, Tanja, Ellis, Julia H., Côrtez-Real, Beatriz, Ciclitira, Paul Jonathan
Format: Article
Language:English
English
Published: Wiley-Blackwell 2016
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Online Access:http://irep.iium.edu.my/54747/9/54747_Coeliac%20disease_article.pdf
http://irep.iium.edu.my/54747/2/54747_Coeliac%20disease_SCOPUS.pdf
http://irep.iium.edu.my/54747/
http://onlinelibrary.wiley.com/journal/10.1111/(ISSN)1365-2613
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spelling my.iium.irep.547472018-05-02T05:55:45Z http://irep.iium.edu.my/54747/ Coeliac disease: immunogenicity studies of barley hordein and rye secalin-derived peptides Abdul Wahab, Widya Šuligoj, Tanja Ellis, Julia H. Côrtez-Real, Beatriz Ciclitira, Paul Jonathan Q Science (General) Coeliac disease (CD) is an inflammatory disorder of the small intestine. It includes aberrant adaptive immunity with presentation of CD toxic gluten peptides by HLA-DQ2 or DQ8 molecules to gluten-sensitive T cells. A ω-gliadin/C-hordein peptide (QPFPQPEQPFPW) and a rye-derived secalin peptide (QPFPQPQQPIPQ) were proposed to be toxic in CD, as they yielded positive responses when assessed with peripheral blood T-cell clones derived from individuals with CD. We sought to assess the immunogenicity of the candidate peptides using gluten-sensitive T-cell lines obtained from CD small intestinal biopsies. We also sought to investigate the potential cross-reactivity of wheat gluten-sensitive T-cell lines with peptic–tryptic digested barley hordein (PTH) and rye secalin (PTS). Synthesised candidate peptides were deamidated with tissue transglutaminase (tTG). Gluten-sensitive T-cell lines were generated by culturing small intestinal biopsies from CD patients with peptic–tryptic gluten (PTG), PTH or PTS, along with autologous PBMCs for antigen presentation. The stimulation indices were determined by measuring the relative cellular proliferation via incorporation of 3H-thymidine. The majority of T-cell lines reacted to the peptides studied. There was also cross-reactivity between wheat gluten-sensitive T-cell lines and the hordein, gliadin and secalin peptides. PTH, PTS, barley hordein and rye secalin-derived CD antigen-sensitive T-cell lines showed positive stimulation with PTG. ω-gliadin/C-hordein peptide and rye-derived peptide are immunogenic to gluten-sensitive T-cell lines and potentially present in wheat, rye and barley. Additional CD toxic peptides may be shared. © 2016 The Authors. International Journal of Experimental Pathology © 2016 International Journal of Experimental Pathology Wiley-Blackwell 2016-08-01 Article REM application/pdf en http://irep.iium.edu.my/54747/9/54747_Coeliac%20disease_article.pdf application/pdf en http://irep.iium.edu.my/54747/2/54747_Coeliac%20disease_SCOPUS.pdf Abdul Wahab, Widya and Šuligoj, Tanja and Ellis, Julia H. and Côrtez-Real, Beatriz and Ciclitira, Paul Jonathan (2016) Coeliac disease: immunogenicity studies of barley hordein and rye secalin-derived peptides. International Journal of Experimental Pathology, 97 (4). pp. 303-309. ISSN 0959-9673 http://onlinelibrary.wiley.com/journal/10.1111/(ISSN)1365-2613 10.1111/iep.12199
institution Universiti Islam Antarabangsa Malaysia
building IIUM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider International Islamic University Malaysia
content_source IIUM Repository (IREP)
url_provider http://irep.iium.edu.my/
language English
English
topic Q Science (General)
spellingShingle Q Science (General)
Abdul Wahab, Widya
Šuligoj, Tanja
Ellis, Julia H.
Côrtez-Real, Beatriz
Ciclitira, Paul Jonathan
Coeliac disease: immunogenicity studies of barley hordein and rye secalin-derived peptides
description Coeliac disease (CD) is an inflammatory disorder of the small intestine. It includes aberrant adaptive immunity with presentation of CD toxic gluten peptides by HLA-DQ2 or DQ8 molecules to gluten-sensitive T cells. A ω-gliadin/C-hordein peptide (QPFPQPEQPFPW) and a rye-derived secalin peptide (QPFPQPQQPIPQ) were proposed to be toxic in CD, as they yielded positive responses when assessed with peripheral blood T-cell clones derived from individuals with CD. We sought to assess the immunogenicity of the candidate peptides using gluten-sensitive T-cell lines obtained from CD small intestinal biopsies. We also sought to investigate the potential cross-reactivity of wheat gluten-sensitive T-cell lines with peptic–tryptic digested barley hordein (PTH) and rye secalin (PTS). Synthesised candidate peptides were deamidated with tissue transglutaminase (tTG). Gluten-sensitive T-cell lines were generated by culturing small intestinal biopsies from CD patients with peptic–tryptic gluten (PTG), PTH or PTS, along with autologous PBMCs for antigen presentation. The stimulation indices were determined by measuring the relative cellular proliferation via incorporation of 3H-thymidine. The majority of T-cell lines reacted to the peptides studied. There was also cross-reactivity between wheat gluten-sensitive T-cell lines and the hordein, gliadin and secalin peptides. PTH, PTS, barley hordein and rye secalin-derived CD antigen-sensitive T-cell lines showed positive stimulation with PTG. ω-gliadin/C-hordein peptide and rye-derived peptide are immunogenic to gluten-sensitive T-cell lines and potentially present in wheat, rye and barley. Additional CD toxic peptides may be shared. © 2016 The Authors. International Journal of Experimental Pathology © 2016 International Journal of Experimental Pathology
format Article
author Abdul Wahab, Widya
Šuligoj, Tanja
Ellis, Julia H.
Côrtez-Real, Beatriz
Ciclitira, Paul Jonathan
author_facet Abdul Wahab, Widya
Šuligoj, Tanja
Ellis, Julia H.
Côrtez-Real, Beatriz
Ciclitira, Paul Jonathan
author_sort Abdul Wahab, Widya
title Coeliac disease: immunogenicity studies of barley hordein and rye secalin-derived peptides
title_short Coeliac disease: immunogenicity studies of barley hordein and rye secalin-derived peptides
title_full Coeliac disease: immunogenicity studies of barley hordein and rye secalin-derived peptides
title_fullStr Coeliac disease: immunogenicity studies of barley hordein and rye secalin-derived peptides
title_full_unstemmed Coeliac disease: immunogenicity studies of barley hordein and rye secalin-derived peptides
title_sort coeliac disease: immunogenicity studies of barley hordein and rye secalin-derived peptides
publisher Wiley-Blackwell
publishDate 2016
url http://irep.iium.edu.my/54747/9/54747_Coeliac%20disease_article.pdf
http://irep.iium.edu.my/54747/2/54747_Coeliac%20disease_SCOPUS.pdf
http://irep.iium.edu.my/54747/
http://onlinelibrary.wiley.com/journal/10.1111/(ISSN)1365-2613
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