Effect of captive breeding on the Amoebocyte cell viability in Malaysian horseshoe crab (Tachypleus gigas)

• Main Authors: Sheikh Mohamed, Hassan Ibrahim, John, Akbar, Yunus, Kamaruzzaman, S., Solahuddin
• Format: Conference or Workshop Item
• Language: English
• Published: Fakulti Sains dan Teknologi, Universiti Kebangsaan Malaysia 2016
• Subjects: QD Chemistry
• Online Access: http://irep.iium.edu.my/54269/1/54269_EFFECT%20OF%20CAPTIVE%20BREEDING.pdf; http://irep.iium.edu.my/54269/; http://msabsimposium.blogspot.my/p/poster-presentation.html

Amoebocyte cells degranulate in response to the bacterial pyrogen, which is the basis for the detection of endotoxin in various medical applications. It is presumed that the captive breeding might impart the cellular reaction by altering their functionality. Crabs under 4 month captivity and wild crabs were bled in triplicate to determine cell viability and density. A total of 3 anticoagulants formulations (A, B and C) were used to obtain the amoebocyte cells. Results showed that the significant reduction in the cell viability of captive crabs compared to the wild crabs (P<0.01). The effect of different anticoagulants on cell viability was also apparent in this study. Cell viability of captive crabs in anticoagulant A, B and C were 40%, 58% and 52% respectively. Whilst, the cell viability of wild crabs was 80%, 86% and 84% respectively. On the contrary, mean cell density in captive crabs were higher compared to the wild crabs (P<0.05) with the mean value of 8×106 cells/ml and 6×106 cells/ml in captive and wild crabs respectively. The performance of different anticoagulants used in this study was in the order of B>C>A (refer methodology). We conclude that the captive breeding has profound negative effect on the amoebocyte viability.